Stimulation of Cultured H9 Human Embryonic Stem Cells with Thyroid Stimulating Hormone Does Not Lead to Formation of Thyroid-Like Cells
المؤلفون المشاركون
Neumann, Ronald D.
Onyshchenko, Mykola I.
Panyutin, Igor G.
Panyutin, Irina V.
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-03-11
دولة النشر
مصر
عدد الصفحات
8
الملخص EN
The sodium-iodine symporter (NIS) is expressed on the cell membrane of many thyroid cancer cells, and is responsible for the radioactive iodine accumulation.
However, treatment of anaplastic thyroid cancer is ineffective due to the low expression of NIS on cell membranes of these tumor cells.
Human embryonic stem cells (ESCs) provide a potential vehicle to study the mechanisms of NIS expression regulation during differentiation.
Human ESCs were maintained on feeder-independent culture conditions.
RT-qPCR and immunocytochemistry were used to study differentiation marker expression, 125I uptake to study NIS function.
We designed a two-step protocol for human ESC differentiation into thyroid-like cells, as was previously done for mouse embryonic stem cells.
First, we obtained definitive endoderm from human ESCs.
Second, we directed differentiation of definitive endoderm cells into thyroid-like cells using various factors, with thyroid stimulating hormone (TSH) as the main differentiating factor.
Expression of pluripotency, endoderm and thyroid markers and 125I uptake were monitored throughout the differentiation steps.
These approaches did not result in efficient induction of thyroid-like cells.
We conclude that differentiation of human ESCs into thyroid cells cannot be induced by TSH media supplementation alone and most likely involves complicated developmental patterns that are yet to be understood.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Onyshchenko, Mykola I.& Panyutin, Igor G.& Panyutin, Irina V.& Neumann, Ronald D.. 2012. Stimulation of Cultured H9 Human Embryonic Stem Cells with Thyroid Stimulating Hormone Does Not Lead to Formation of Thyroid-Like Cells. Stem Cells International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1003063
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Onyshchenko, Mykola I.…[et al.]. Stimulation of Cultured H9 Human Embryonic Stem Cells with Thyroid Stimulating Hormone Does Not Lead to Formation of Thyroid-Like Cells. Stem Cells International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-1003063
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Onyshchenko, Mykola I.& Panyutin, Igor G.& Panyutin, Irina V.& Neumann, Ronald D.. Stimulation of Cultured H9 Human Embryonic Stem Cells with Thyroid Stimulating Hormone Does Not Lead to Formation of Thyroid-Like Cells. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-1003063
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1003063
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر