Temporal Requirements of cMyc Protein for Reprogramming Mouse Fibroblasts

المؤلفون المشاركون

Sumer, Huseyin
Verma, Paul J.
Heffernan, Corey
Malaver-Ortega, Luis F.

المصدر

Stem Cells International

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-12، 12ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2012-04-24

دولة النشر

مصر

عدد الصفحات

12

الملخص EN

Exogenous expression of Oct4, Sox2, Klf4, and cMyc forces mammalian somatic cells to adopt molecular and phenotypic characteristics of embryonic stem cells, commencing with the required suppression of lineage-associated genes (e.g., Thy1 in mouse).

Although omitting cMyc from the reprogramming cocktail minimizes risks of uncontrolled proliferation, its exclusion results in fold reductions in reprogramming efficiency.

Thus, the feasibility of substituting cMyc transgene with (non-integrative) recombinant “pTAT-mcMyc” protein delivery was assessed, without compromising reprogramming efficiency or the pluripotent phenotype.

Purification and delivery of semisoluble/particulate pTAT-mcMyc maintained Oct4-GFP+ colony formation (i.e., reprogramming efficiency) whilst supporting pluripotency by various criteria.

Differential repression of Thy1 by pTAT-mcMyc ± Oct4, Sox2, and Klf4 (OSK) suggested differential (and non-additive) mechanisms of repression.

Extending these findings, attempts to enhance reprogramming efficiency through a staggered approach (prerepression of Thy1) failed to improve reprogramming efficiency.

We consider protein delivery a useful tool to decipher temporal/molecular events characterizing somatic cell reprogramming.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Heffernan, Corey& Sumer, Huseyin& Malaver-Ortega, Luis F.& Verma, Paul J.. 2012. Temporal Requirements of cMyc Protein for Reprogramming Mouse Fibroblasts. Stem Cells International،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1030028

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Heffernan, Corey…[et al.]. Temporal Requirements of cMyc Protein for Reprogramming Mouse Fibroblasts. Stem Cells International No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-1030028

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Heffernan, Corey& Sumer, Huseyin& Malaver-Ortega, Luis F.& Verma, Paul J.. Temporal Requirements of cMyc Protein for Reprogramming Mouse Fibroblasts. Stem Cells International. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-1030028

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1030028