The Effect of TLR9 Agonist CpG Oligodeoxynucleotides on the Intestinal Immune Response of Cobia (Rachycentron canadum)
المؤلفون المشاركون
Cheng, Ta Chih
Byadgi, Omkar
Puteri, Dinda
Lee, Jai-Wei
Chang, Tsung-Chou
Lee, Yan-Horn
Chu, Chun-Yen
المصدر
Journal of Immunology Research
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-02
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Cytosine-guanine oligodeoxynucleotide (CpG ODN) motifs of bacterial DNA are recognized through toll-like receptor 9 (TLR9) and are potent activators of innate immunity.
However, the interaction between TLR9 and CpG ODN in aquatic species has not been well characterized.
Hence, cobia TLR9 isoform B (RCTLR9B) was cloned and its expression and induction in intestine were investigated.
RCTLR9B cDNA consists of 3113bp encoding 1009 amino acids containing three regions, leucine rich repeats, transmembrane domain, and toll/interleukin-1 receptor (TIR) domain.
Intraperitoneal injection of CpG ODN 2395 upregulated RCTLR9 A and B and MyD88 and also induced the expressions of Mx, chemokine CC, and interleukin IL-1β.
Cobia intraperitoneally injected with CpG ODN 1668 and 2395 had increased survival rates after challenge with Photobacterium damselae subsp.
piscicida.
In addition, formulation of CpG ODN with formalin-killed bacteria (FKB) and aluminum hydroxide gel significantly increased expressions of RCTLR9 A (50 folds) and B (30 folds) isoforms at 10 dpi (CpG ODN 1668) and MyD88 (21 folds) at 6 dpv (CpG ODN 2395).
Subsequently, IL-1β increased at 6 dpv in 1668 group.
No histopathological damage and inflammatory responses were observed in the injected cobia.
Altogether, these results facilitate CpG ODNs as an adjuvant to increase bacterial disease resistance and efficacy of vaccines in cobia.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Byadgi, Omkar& Puteri, Dinda& Lee, Jai-Wei& Chang, Tsung-Chou& Lee, Yan-Horn& Chu, Chun-Yen…[et al.]. 2014. The Effect of TLR9 Agonist CpG Oligodeoxynucleotides on the Intestinal Immune Response of Cobia (Rachycentron canadum). Journal of Immunology Research،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1040809
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Byadgi, Omkar…[et al.]. The Effect of TLR9 Agonist CpG Oligodeoxynucleotides on the Intestinal Immune Response of Cobia (Rachycentron canadum). Journal of Immunology Research No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-1040809
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Byadgi, Omkar& Puteri, Dinda& Lee, Jai-Wei& Chang, Tsung-Chou& Lee, Yan-Horn& Chu, Chun-Yen…[et al.]. The Effect of TLR9 Agonist CpG Oligodeoxynucleotides on the Intestinal Immune Response of Cobia (Rachycentron canadum). Journal of Immunology Research. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-1040809
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1040809
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر