Krüppel-Like Factor 5 Mediates Proinflammatory Cytokine Expression in Lipopolysaccharide-Induced Acute Lung Injury through Upregulation of Nuclear Factor- κB Phosphorylation In Vitro and In Vivo
المؤلفون المشاركون
Liu, Po-Len
Wang, Hui-Min
Chen, Hsiu-Lin
Chong, Inn-Wen
Lee, Yi-Chen
Tsai, Jong-Rung
Yuan, Shyng-Shiou F.
Liu, Wei-Lun
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-10
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Acute lung injury (ALI) is associated with an inflammation-mediated process, and the transcription factor, Krüppel-like factor 5 (KLF5), might play a crucial role in inflammatory lung disease.
In this study, we evaluated KLF5, reactive oxygen species (ROS), and inflammatory responses in a lipopolysaccharide- (LPS-) induced ALI model to elucidate the role of KLF5 in ALI.
Our data indicated that LPS upregulates proinflammatory cytokine expression in human bronchial epithelial cells in a dose-dependent manner.
We observed upregulated KLF5 protein expression in human bronchial epithelial cells exposed to LPS, with peak expression 1 h after LPS treatment, and subsequent upregulation of p65 protein expression and p65 phosphorylation at Ser276.
These results indicate that KLF5 mediates proinflammatory cytokine expression by upregulating nuclear factor-kappaB (NF-κB) phosphorylation at p65 in response to LPS.
LPS treatment also increased ROS production and simultaneously upregulated KLF5 expression and NF-κB translocation.
N-acetylcysteine significantly reduced ROS levels and KLF5 and NF-κB translocation in nuclear extracts.
Therefore, N-acetylcysteine pretreatment before LPS exposure reduces ROS, downregulates KLF5 expression, and subsequently reduces inflammatory responses by scavenging ROS.
Overall, our study results indicate that KLF5 mediates proinflammatory cytokine expression through upregulation of NF-κB phosphorylation at p65 in LPS-induced ALI.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Hsiu-Lin& Chong, Inn-Wen& Lee, Yi-Chen& Tsai, Jong-Rung& Yuan, Shyng-Shiou F.& Wang, Hui-Min…[et al.]. 2014. Krüppel-Like Factor 5 Mediates Proinflammatory Cytokine Expression in Lipopolysaccharide-Induced Acute Lung Injury through Upregulation of Nuclear Factor- κB Phosphorylation In Vitro and In Vivo. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043418
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Hsiu-Lin…[et al.]. Krüppel-Like Factor 5 Mediates Proinflammatory Cytokine Expression in Lipopolysaccharide-Induced Acute Lung Injury through Upregulation of Nuclear Factor- κB Phosphorylation In Vitro and In Vivo. Mediators of Inflammation No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1043418
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Hsiu-Lin& Chong, Inn-Wen& Lee, Yi-Chen& Tsai, Jong-Rung& Yuan, Shyng-Shiou F.& Wang, Hui-Min…[et al.]. Krüppel-Like Factor 5 Mediates Proinflammatory Cytokine Expression in Lipopolysaccharide-Induced Acute Lung Injury through Upregulation of Nuclear Factor- κB Phosphorylation In Vitro and In Vivo. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043418
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1043418
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر