MicroRNA-146a Decreases High GlucoseThrombin-Induced Endothelial Inflammation by Inhibiting NAPDH Oxidase 4 Expression
المؤلفون المشاركون
Peng, Ching-Tien
Wang, Huang-Joe
Huang, Yuan-Li
Shih, Ya-Yun
Wu, Hsing-Yu
Lo, Wan-Yu
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-09-14
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Diabetes is associated with hyperglycemia and increased thrombin production.
However, it is unknown whether a combination of high glucose and thrombin can modulate the expression of NAPDH oxidase (Nox) subtypes in human aortic endothelial cells (HAECs).
Moreover, we investigated the role of a diabetes-associated microRNA (miR-146a) in a diabetic atherothrombosis model.
We showed that high glucose (HG) exerted a synergistic effect with thrombin to induce a 10.69-fold increase in Nox4 mRNA level in HAECs.
Increased Nox4 mRNA expression was associated with increased Nox4 protein expression and ROS production.
Inflammatory cytokine kit identified that the treatment increased IL-8 and IL-6 levels.
Moreover, HG/thrombin treatment caused an 11.43-fold increase of THP-1 adhesion to HAECs.
In silico analysis identified the homology between miR-146a and the 3′-untranslated region of the Nox4 mRNA, and a luciferase reporter assay confirmed that the miR-146a mimic bound to this Nox4 regulatory region.
Additionally, miR-146a expression was decreased to 58% of that in the control, indicating impaired feedback restraint of HG/thrombin-induced endothelial inflammation.
In contrast, miR-146a mimic transfection attenuated HG/thrombin-induced upregulation of Nox4 expression, ROS generation, and inflammatory phenotypes.
In conclusion, miR-146a is involved in the regulation of endothelial inflammation via modulation of Nox4 expression in a diabetic atherothrombosis model.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Huang-Joe& Huang, Yuan-Li& Shih, Ya-Yun& Wu, Hsing-Yu& Peng, Ching-Tien& Lo, Wan-Yu. 2014. MicroRNA-146a Decreases High GlucoseThrombin-Induced Endothelial Inflammation by Inhibiting NAPDH Oxidase 4 Expression. Mediators of Inflammation،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043514
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Huang-Joe…[et al.]. MicroRNA-146a Decreases High GlucoseThrombin-Induced Endothelial Inflammation by Inhibiting NAPDH Oxidase 4 Expression. Mediators of Inflammation No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1043514
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Huang-Joe& Huang, Yuan-Li& Shih, Ya-Yun& Wu, Hsing-Yu& Peng, Ching-Tien& Lo, Wan-Yu. MicroRNA-146a Decreases High GlucoseThrombin-Induced Endothelial Inflammation by Inhibiting NAPDH Oxidase 4 Expression. Mediators of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1043514
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1043514
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر