Differentiation of Equine Mesenchymal Stromal Cells into Cells of Neural Lineage: Potential for Clinical Applications
المؤلفون المشاركون
Dhar, M. S.
Amelse, Lisa
Neilsen, Nancy
Dunlap, John R.
Cruz Villagrán, Claudia
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-11-24
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Mesenchymal stromal cells (MSCs) are able to differentiate into extramesodermal lineages, including neurons.
Positive outcomes were obtained after transplantation of neurally induced MSCs in laboratory animals after nerve injury, but this is unknown in horses.
Our objectives were to test the ability of equine MSCs to differentiate into cells of neural lineage in vitro, to assess differences in morphology and lineage-specific protein expression, and to investigate if horse age and cell passage number affected the ability to achieve differentiation.
Bone marrow-derived MSCs were obtained from young and adult horses.
Following demonstration of stemness, MSCs were neurally induced and microscopically assessed at different time points.
Results showed that commercially available nitrogen-coated tissue culture plates supported proliferation and differentiation.
Morphological changes were immediate and all the cells displayed a neural crest-like cell phenotype.
Expression of neural progenitor proteins, was assessed via western blot or immunofluorescence.
In our study, MSCs generated from young and middle-aged horses did not show differences in their ability to undergo differentiation.
The effect of cell passage number, however, is inconsistent and further experiments are needed.
Ongoing work is aimed at transdifferentiating these cells into Schwann cells for transplantation into a peripheral nerve injury model in horses.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cruz Villagrán, Claudia& Amelse, Lisa& Neilsen, Nancy& Dunlap, John R.& Dhar, M. S.. 2014. Differentiation of Equine Mesenchymal Stromal Cells into Cells of Neural Lineage: Potential for Clinical Applications. Stem Cells International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1047535
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cruz Villagrán, Claudia…[et al.]. Differentiation of Equine Mesenchymal Stromal Cells into Cells of Neural Lineage: Potential for Clinical Applications. Stem Cells International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-1047535
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cruz Villagrán, Claudia& Amelse, Lisa& Neilsen, Nancy& Dunlap, John R.& Dhar, M. S.. Differentiation of Equine Mesenchymal Stromal Cells into Cells of Neural Lineage: Potential for Clinical Applications. Stem Cells International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-1047535
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1047535
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر