Integrative Analysis of CRISPRCas9 Target Sites in the Human HBB Gene
المؤلفون المشاركون
Luo, Yumei
Zhu, Detu
Pan, Shen Quan
Zhang, Zhizhuo
Chen, Yaoyong
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-31
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Recently, the clustered regularly interspaced short palindromic repeats (CRISPR) system has emerged as a powerful customizable artificial nuclease to facilitate precise genetic correction for tissue regeneration and isogenic disease modeling.
However, previous studies reported substantial off-target activities of CRISPR system in human cells, and the enormous putative off-target sites are labor-intensive to be validated experimentally, thus motivating bioinformatics methods for rational design of CRISPR system and prediction of its potential off-target effects.
Here, we describe an integrative analytical process to identify specific CRISPR target sites in the human β-globin gene (HBB) and predict their off-target effects.
Our method includes off-target analysis in both coding and noncoding regions, which was neglected by previous studies.
It was found that the CRISPR target sites in the introns have fewer off-target sites in the coding regions than those in the exons.
Remarkably, target sites containing certain transcriptional factor motif have enriched binding sites of relevant transcriptional factor in their off-target sets.
We also found that the intron sites have fewer SNPs, which leads to less variation of CRISPR efficiency in different individuals during clinical applications.
Our studies provide a standard analytical procedure to select specific CRISPR targets for genetic correction.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Luo, Yumei& Zhu, Detu& Zhang, Zhizhuo& Chen, Yaoyong& Pan, Shen Quan. 2015. Integrative Analysis of CRISPRCas9 Target Sites in the Human HBB Gene. BioMed Research International،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055777
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Luo, Yumei…[et al.]. Integrative Analysis of CRISPRCas9 Target Sites in the Human HBB Gene. BioMed Research International No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1055777
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Luo, Yumei& Zhu, Detu& Zhang, Zhizhuo& Chen, Yaoyong& Pan, Shen Quan. Integrative Analysis of CRISPRCas9 Target Sites in the Human HBB Gene. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1055777
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1055777
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر