Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts
المؤلفون المشاركون
Stoddart, Martin
Voss, Jan O.
Loebel, Claudia
Bara, Jennifer J.
Fussinger, Marc Anton
Alini, Mauro
Duttenhoefer, Fabian
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-22
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Introduction.
Human mesenchymal stromal cells (hMSCs) exhibit the potential to accelerate bone healing by enhanced osteogenic differentiation.
Interleukin-1β is highly expressed during fracture healing and has been demonstrated to exert a significant impact on the differentiation behaviour of hMSCs.
Here, we investigate the effect of 2-hour IL-1β stimulation on the differentiation and paracrine activity of hMSCs in coculture with osteosarcoma cells in vitro.
Methods.
hMSCs from 3 donors were incubated for 2 hours with 10 ng/mL IL-1β and subsequently cocultured with MG63-GFP cells either in control or in differentiation medium in a transwell system for 28 days.
Genetic and functional effects were investigated.
Results.
hMSCs cultured in control medium exhibited a regulatory effect on cocultured MG63-GFP cells, resulting in upregulation of osteogenic gene expression in combination with increased ALP activity.
However, while stimulated hMSCs cultured under differentiation conditions exhibit signs of osteogenic differentiation, osteogenic differentiation also caused an impaired regulatory effect on the cocultured MG63-GFP cells.
Conclusion.
Short stimulation of hMSCs has the potential to modify their long-term behaviour.
In addition, undifferentiated hMSCs are able to regulate osteoblast differentiation; however, this regulatory function is lost upon osteogenic differentiation in vitro.
This offers a novel approach for clinical cell therapy protocols.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Voss, Jan O.& Loebel, Claudia& Bara, Jennifer J.& Fussinger, Marc Anton& Duttenhoefer, Fabian& Alini, Mauro…[et al.]. 2015. Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts. BioMed Research International،Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1056472
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Voss, Jan O.…[et al.]. Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts. BioMed Research International No. 2015 (2015), pp.1-16.
https://search.emarefa.net/detail/BIM-1056472
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Voss, Jan O.& Loebel, Claudia& Bara, Jennifer J.& Fussinger, Marc Anton& Duttenhoefer, Fabian& Alini, Mauro…[et al.]. Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-16.
https://search.emarefa.net/detail/BIM-1056472
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1056472
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر