Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors
المؤلفون المشاركون
Sakkhachornphop, Supachai
Thongkum, Weeraya
Tayapiwatana, Chatchai
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-07
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
The 3′-end processing (3′P) of each viral long terminal repeat (LTR) during human immunodeficiency virus type-1 (HIV-1) integration is a vital step in the HIV life cycle.
Blocking the 3′P using 3′P inhibitor has recently become an attractive strategy for HIV-1 therapeutic intervention.
Recently, we have developed a novel real-time PCR based assay for the detection of 3′P activity in vitro.
The methodology usually involves biotinylated HIV-1 LTR, HIV-1 integrase (IN), and specific primers and probe.
In this novel assay, we designed the HIV-1 LTR substrate based on a sequence with a homology to HIV-1 LTR labeled at its 3′ end with biotin on the sense strand.
Two nucleotides at the 3′ end were subsequently removed by IN activity.
Only two nucleotides labeled biotin were captured on an avidin-coated tube; therefore, inhibiting the binding of primers and probe results in late signals in the real-time PCR.
This novel assay has successfully detected both the 3′P activity of HIV-1 IN and the anti-IN activity by Raltegravir and sodium azide agent.
This real-time PCR assay has been shown to be effective and inexpensive for a high-throughput screening of novel IN inhibitors.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sakkhachornphop, Supachai& Thongkum, Weeraya& Tayapiwatana, Chatchai. 2015. Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors. BioMed Research International،Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1057014
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sakkhachornphop, Supachai…[et al.]. Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors. BioMed Research International No. 2015 (2015), pp.1-7.
https://search.emarefa.net/detail/BIM-1057014
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sakkhachornphop, Supachai& Thongkum, Weeraya& Tayapiwatana, Chatchai. Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors. BioMed Research International. 2015. Vol. 2015, no. 2015, pp.1-7.
https://search.emarefa.net/detail/BIM-1057014
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1057014
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر