Oleanolic Acid Attenuates Insulin Resistance via NF-κB to Regulate the IRS1-GLUT4 Pathway in HepG2 Cells
المؤلفون المشاركون
Bei, Wei-jian
Li, Ming
Han, Zongyu
Rong, Xianglu
Guo, Jiao
Hu, Xuguang
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-30
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The aim of our study is to elucidate the mechanisms of oleanolic acid (OA) on insulin resistance (IR) in HepG2 cells.
HepG2 cells were induced with FFA as the insulin resistance model and were treated with OA.
Then the glucose content and the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were analyzed.
Moreover, protein expression of nuclear factor kappa B (NF-κB), insulin receptor substrate 1(IRS1), and glucose transporter 4 (GLUT4) in cells treated with OA were measured by Western blot analysis.
Additionally, IRS1 protein expression exposed to OA was detected after using pyrrolidine dithiocarbamate (PDTC).Our results revealed that OA decreased the glucose content in HepG2 cells in vitro.
Moreover, OA reduced the levels of TNF-α and IL-6 and upregulated IRS1 and GLUT4 protein expression.
Furthermore, OA also reduced NF-κB protein expression in insulin-resistant HepG2 cells.
After blocking NF-κB, the expression of IRS1 protein had no obvious changes when treated with OA.
OA attenuated insulin resistance and decreased the levels of TNF-α and IL-6.
Meanwhile, OA decreased NF-κB protein expression and upregulated IRS1 and GLUT4 protein expression.
Therefore, regulating the IRS1-GLUT4 pathway via NF-κB was the underlying mechanism of OA on insulin resistance.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Ming& Han, Zongyu& Bei, Wei-jian& Rong, Xianglu& Guo, Jiao& Hu, Xuguang. 2015. Oleanolic Acid Attenuates Insulin Resistance via NF-κB to Regulate the IRS1-GLUT4 Pathway in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063424
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Ming…[et al.]. Oleanolic Acid Attenuates Insulin Resistance via NF-κB to Regulate the IRS1-GLUT4 Pathway in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1063424
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Ming& Han, Zongyu& Bei, Wei-jian& Rong, Xianglu& Guo, Jiao& Hu, Xuguang. Oleanolic Acid Attenuates Insulin Resistance via NF-κB to Regulate the IRS1-GLUT4 Pathway in HepG2 Cells. Evidence-Based Complementary and Alternative Medicine. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1063424
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1063424
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر