CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a
المؤلفون المشاركون
Vaivoda, Rachel
Vaine, Christine
Boerstler, Cassandra
Galloway, Kristy
Christmas, Peter
المصدر
Journal of Immunology Research
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-03
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
CYP4Fs were first identified as enzymes that catalyze hydroxylation of leukotriene B4 (LTB4).
CYP4F18 has an unusual expression in neutrophils and was predicted to play a role in regulating LTB4-dependent inflammation.
We compared chemotaxis of wild-type and Cyp4f18 knockout neutrophils using an in vitro assay.
There was no significant difference in the chemotactic response to LTB4, but the response to complement component C5a increased 1.9–2.25-fold in knockout cells compared to wild-type (P < 0.01).
This increase was still observed when neutrophils were treated with inhibitors of eicosanoid synthesis.
There were no changes in expression of other CYP4 enzymes in knockout neutrophils that might compensate for loss of CYP4F18 or lead to differences in activity.
A mouse model of dextran sodium sulfate colitis was used to investigate the consequences of increased C5a-dependent chemotaxis in vivo, but there was no significant difference in weight loss, disease activity, or colonic tissue myeloperoxidase between wild-type and Cyp4f18 knockout mice.
This study demonstrates the limitations of inferring CYP4F function based on an ability to use LTB4 as a substrate, points to expanding roles for CYP4F enzymes in immune regulation, and underscores the in vivo challenges of CYP knockout studies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Vaivoda, Rachel& Vaine, Christine& Boerstler, Cassandra& Galloway, Kristy& Christmas, Peter. 2015. CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068417
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Vaivoda, Rachel…[et al.]. CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a. Journal of Immunology Research No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1068417
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Vaivoda, Rachel& Vaine, Christine& Boerstler, Cassandra& Galloway, Kristy& Christmas, Peter. CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1068417
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1068417
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر