In Vitro and In Vivo Comparison of Lymphocytes Transduced with a Human CD16 or with a Chimeric Antigen Receptor Reveals Potential Off-Target Interactions due to the IgG2 CH2-CH3 CAR-Spacer
المؤلفون المشاركون
Clémenceau, Béatrice
Valsesia-Wittmann, Sandrine
Jallas, Anne-Catherine
Vivien, Régine
Rousseau, Raphaël
Marabelle, Aurélien
Caux, Christophe
Vié, Henri
المصدر
Journal of Immunology Research
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-17
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
The present work was designed to compare two mechanisms of cellular recognition based on Ab specificity: firstly, when the anti-HER2 mAb trastuzumab bridges target cells and cytotoxic lymphocytes armed with a Fc receptor (ADCC) and, secondly, when HER2 positive target cells are directly recognized by cytotoxic lymphocytes armed with a chimeric antigen receptor (CAR).
To compare these two mechanisms, we used the same cellular effector (NK-92) and the same signaling domain (FcεRIγ).
The NK-92 cytotoxic cell line was transfected with either a FcγRIIIa-FcεRIγ (NK-92CD16) or a trastuzumab-based scFv-FcεRIγ chimeric receptor (NK-92CAR).
In vitro, the cytotoxic activity against HER2 positive target cells after indirect recognition by NK-92CD16 was always inferior to that observed after direct recognition by NK-92CAR.
In contrast, and somehow unexpectedly, in vivo, adoptive transfer of NK-92CD16 + trastuzumab but not of NK-92CAR induced tumor regression.
Analysis of the in vivo xenogeneic system suggested that the human CH2-CH3 IgG2 used as a spacer in our construct was able to interact with the FcR present at the cell surface of the few NSG-FcR+ remaining immune cells.
This interaction, leading to blockage of the NK-92CAR in the periphery of the engrafted tumor cells, stresses the critical role of the composition of the spacer domain.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Clémenceau, Béatrice& Valsesia-Wittmann, Sandrine& Jallas, Anne-Catherine& Vivien, Régine& Rousseau, Raphaël& Marabelle, Aurélien…[et al.]. 2015. In Vitro and In Vivo Comparison of Lymphocytes Transduced with a Human CD16 or with a Chimeric Antigen Receptor Reveals Potential Off-Target Interactions due to the IgG2 CH2-CH3 CAR-Spacer. Journal of Immunology Research،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1068490
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Clémenceau, Béatrice…[et al.]. In Vitro and In Vivo Comparison of Lymphocytes Transduced with a Human CD16 or with a Chimeric Antigen Receptor Reveals Potential Off-Target Interactions due to the IgG2 CH2-CH3 CAR-Spacer. Journal of Immunology Research No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1068490
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Clémenceau, Béatrice& Valsesia-Wittmann, Sandrine& Jallas, Anne-Catherine& Vivien, Régine& Rousseau, Raphaël& Marabelle, Aurélien…[et al.]. In Vitro and In Vivo Comparison of Lymphocytes Transduced with a Human CD16 or with a Chimeric Antigen Receptor Reveals Potential Off-Target Interactions due to the IgG2 CH2-CH3 CAR-Spacer. Journal of Immunology Research. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1068490
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1068490
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر