Electrospun Gelatinβ-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling
المؤلفون المشاركون
Zhang, Xuehui
Meng, Song
Wei, Yan
Xu, Mingming
Huang, Ying
He, Ying
Lin, Hong
Han, Jianmin
Chai, Yuan
Liang, Xu-Deng
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-06-01
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
Calcium phosphate- (CaP-) based composite scaffolds have been used extensively for the bone regeneration in bone tissue engineering.
Previously, we developed a biomimetic composite nanofibrous membrane of gelatin/β-tricalcium phosphate (TCP) and confirmed their biological activity in vitro and bone regeneration in vivo.
However, how these composite nanofibers promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is unknown.
Here, gelatin/β-TCP composite nanofibers were fabricated by incorporating 20 wt% β-TCP nanoparticles into electrospun gelatin nanofibers.
Electron microscopy showed that the composite β-TCP nanofibers had a nonwoven structure with a porous network and a rough surface.
Spectral analyses confirmed the presence and chemical stability of the β-TCP and gelatin components.
Compared with pure gelatin nanofibers, gelatin/β-TCP composite nanofibers caused increased cell attachment, proliferation, alkaline phosphatase activity, and osteogenic gene expression in rat BMSCs.
Interestingly, the expression level of the calcium-sensing receptor (CaSR) was significantly higher on the composite nanofibrous scaffolds than on pure gelatin.
For rat calvarial critical sized defects, more extensive osteogenesis and neovascularization occurred in the composite scaffolds group compared with the gelatin group.
Thus, gelatin/β-TCP composite scaffolds promote osteogenic differentiation of BMSCs in vitro and bone regeneration in vivo by activating Ca2+-sensing receptor signaling.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Xuehui& Meng, Song& Huang, Ying& Xu, Mingming& He, Ying& Lin, Hong…[et al.]. 2015. Electrospun Gelatinβ-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling. Stem Cells International،Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1076233
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Xuehui…[et al.]. Electrospun Gelatinβ-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling. Stem Cells International No. 2015 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1076233
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Xuehui& Meng, Song& Huang, Ying& Xu, Mingming& He, Ying& Lin, Hong…[et al.]. Electrospun Gelatinβ-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-13.
https://search.emarefa.net/detail/BIM-1076233
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1076233
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر