Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study
المؤلفون المشاركون
Henrich, Birgit
Hermann, Ilka
Di Giulio, Mara
Sivalingam, Sugi
Peters, Ulrike
Beikler, Thomas
Janda, Ralf
Rüttermann, Stefan
Köhrer, Karl
Deenen, René
المصدر
Advances in Materials Science and Engineering
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-07-13
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Purpose.
To introduce additional methods to detect and to quantify single pathogens in the complex biofilm formation on an antibacterial dental material.
Materials and Methods.
A conventional (ST) and an antibacterial dental composite (B) were manufactured.
In vitro: specimens were incubated with a mixture of early colonizers.
Bacterial adhesion was analyzed by TaqMan PCR after 8/24 h.
In situ: TaqMan PCR and 16S rRNA Next Generation Sequencing (NGS) were performed.
Results.
In vitro: after 8 h incubation, B was covered by 58.6% of the bacterial amount that was attached to ST.
After 24 h, the amount of attached bacteria to ST remained constant on ST only slightly lower on B.
In situ: after 8 h the amount of adhering A.
viscosus and S.
mitis was prominent on ST and reduced on B.
NGS revealed that S.
sanguinis, S.
parasanguinis, and Gemella sanguinis were the mainly attached species with S.
sanguinis dominant on ST and S.
parasanguinis and G.
sanguinis dominant on B.
Conclusions.
Initial biofilm formation was altered by B.
A shift between actinomycetes and streptococci was observed in situ.
TaqMan PCR and 16S rRNA NGS revealed comparable results in situ and demonstrated the usefulness of NGS to characterize complex bacterial communities.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Henrich, Birgit& Hermann, Ilka& Di Giulio, Mara& Köhrer, Karl& Deenen, René& Sivalingam, Sugi…[et al.]. 2016. Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study. Advances in Materials Science and Engineering،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096277
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Henrich, Birgit…[et al.]. Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study. Advances in Materials Science and Engineering No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1096277
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Henrich, Birgit& Hermann, Ilka& Di Giulio, Mara& Köhrer, Karl& Deenen, René& Sivalingam, Sugi…[et al.]. Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study. Advances in Materials Science and Engineering. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096277
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1096277
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر