Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay
المؤلفون المشاركون
Adewumi, Moses Olubusuyi
Faleye, Temitope Oluwasegun Cephas
Coker, Bamidele Atinuke
Nudamajo, Felix Yasha
Adeniji, Johnson Adekunle
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-03-20
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Recently, a cell-culture independent protocol for detection of enteroviruses from clinical specimen was recommended by the WHO for surveillance alongside the previously established protocols.
Here, we investigated whether this new protocol will show the same enterovirus diversity landscape as the established cell-culture dependent protocols.
Faecal samples were collected from sixty apparently healthy children in Ibadan, Nigeria.
Samples were resuspended in phosphate buffered saline, RNA was extracted, and the VP1 gene was amplified using WHO recommended RT-snPCR protocol.
Amplicons were sequenced and sequences subjected to phylogenetic analysis.
Fifteen (25%) of the 60 samples yielded the expected band size.
Of the 15 amplicons sequenced, 12 were exploitable.
The remaining 3 had electropherograms with multiple peaks and were unexploitable.
Eleven of the 12 exploitable sequences were identified as Coxsackievirus A1 (CVA1), CVA3, CVA4, CVA8, CVA20, echovirus 32 (E32), enterovirus 71 (EV71), EVB80, and EVC99.
Subsequently, the last exploitable sequence was identified as enterobacteriophage baseplate gene by nucleotide BLAST.
The results of this study document the first description of molecular sequence data on CVA1, CVA8, and E32 strains present in Nigeria.
The result further showed that species A enteroviruses were more commonly detected in the region when cell-culture bias is bypassed.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Faleye, Temitope Oluwasegun Cephas& Adewumi, Moses Olubusuyi& Coker, Bamidele Atinuke& Nudamajo, Felix Yasha& Adeniji, Johnson Adekunle. 2016. Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay. Advances in Virology،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096770
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Faleye, Temitope Oluwasegun Cephas…[et al.]. Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay. Advances in Virology No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1096770
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Faleye, Temitope Oluwasegun Cephas& Adewumi, Moses Olubusuyi& Coker, Bamidele Atinuke& Nudamajo, Felix Yasha& Adeniji, Johnson Adekunle. Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay. Advances in Virology. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096770
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1096770
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر