High Glucose-Induced PC12 Cell Death by Increasing Glutamate Production and Decreasing Methyl Group Metabolism
المؤلفون المشاركون
Chen, Minjiang
Wei, Tingting
Wang, Dan
Xia, Huanhuan
Zhao, Liangcai
Ji, Jiansong
Zheng, Hong
Gao, Hong-Chang
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-19
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Objective.
High glucose- (HG-) induced neuronal cell death is responsible for the development of diabetic neuropathy.
However, the effect of HG on metabolism in neuronal cells is still unclear.
Materials and Methods.
The neural-crest derived PC12 cells were cultured for 72 h in the HG (75 mM) or control (25 mM) groups.
We used NMR-based metabolomics to examine both intracellular and extracellular metabolic changes in HG-treated PC12 cells.
Results.
We found that the reduction in intracellular lactate may be due to excreting more lactate into the extracellular medium under HG condition.
HG also induced the changes of other energy-related metabolites, such as an increased succinate and creatine phosphate.
Our results also reveal that the synthesis of glutamate from the branched-chain amino acids (isoleucine and valine) may be enhanced under HG.
Increased levels of intracellular alanine, phenylalanine, myoinositol, and choline were observed in HG-treated PC12 cells.
In addition, HG-induced decreases in intracellular dimethylamine, dimethylglycine, and 3-methylhistidine may indicate a downregulation of methyl group metabolism.
Conclusions.
Our metabolomic results suggest that HG-induced neuronal cell death may be attributed to a series of metabolic changes, involving energy metabolism, amino acids metabolism, osmoregulation and membrane metabolism, and methyl group metabolism.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Minjiang& Zheng, Hong& Wei, Tingting& Wang, Dan& Xia, Huanhuan& Zhao, Liangcai…[et al.]. 2016. High Glucose-Induced PC12 Cell Death by Increasing Glutamate Production and Decreasing Methyl Group Metabolism. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097671
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Minjiang…[et al.]. High Glucose-Induced PC12 Cell Death by Increasing Glutamate Production and Decreasing Methyl Group Metabolism. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1097671
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Minjiang& Zheng, Hong& Wei, Tingting& Wang, Dan& Xia, Huanhuan& Zhao, Liangcai…[et al.]. High Glucose-Induced PC12 Cell Death by Increasing Glutamate Production and Decreasing Methyl Group Metabolism. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097671
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1097671
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر