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Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection
المؤلفون المشاركون
Lim, Mi Suk
Kim, Eui Chong
Park, Kyoung Un
Hong, Yun Ji
Kim, Taek Soo
Hwang, Sang Mee
Song, Junghan
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-21
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Human papillomavirus (HPV) infection is an important etiologic factor in cervical carcinogenesis.
Various HPV DNA detection methods have been evaluated for clinicopathological level.
For the specimens with normal cytological finding, discrepancies among the detection methods were frequently found and adequate interpretation can be difficult.
6,322 clinical specimens were submitted and evaluated for real-time PCR and Hybrid Capture 2 (HC2).
573 positive or “Not Detected but Amplified” (NDBA) specimens by real-time PCR were additionally tested using genetic analyzer.
For the reliability of real-time PCR, 325 retests were performed.
Optimal cut-off cycle threshold (CT) value was evaluated also.
78.7% of submitted specimens showed normal or nonspecific cytological finding.
The distributions of HPV types by real-time PCR were not different between positive and NDBA cases.
For positive cases by fragment analysis, concordance rates with real-time PCR and HC2 were 94.2% and 84.2%.
In NDBA cases, fragment analysis and real-time PCR showed identical results in 77.0% and HC2 revealed 27.6% of concordance with fragment analysis.
Optimal cut-off CT value was different for HPV types.
NDBA results in real-time PCR should be regarded as equivocal, not negative.
The adjustment of cut-off CT value for HPV types will be helpful for the appropriate result interpretation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kim, Taek Soo& Lim, Mi Suk& Hong, Yun Ji& Hwang, Sang Mee& Park, Kyoung Un& Song, Junghan…[et al.]. 2016. Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection. BioMed Research International،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098016
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kim, Taek Soo…[et al.]. Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection. BioMed Research International No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1098016
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kim, Taek Soo& Lim, Mi Suk& Hong, Yun Ji& Hwang, Sang Mee& Park, Kyoung Un& Song, Junghan…[et al.]. Significance of “Not Detected but Amplified” Results by Real-Time PCR Method for HPV DNA Detection. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1098016
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1098016
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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