A Novel In Vitro System for Comparative Analyses of Bone Cells and Bacteria under Electrical Stimulation
المؤلفون المشاركون
Dauben, Thomas Josef
Ziebart, Josefin
Bender, Thomas
Zaatreh, Sarah
Bader, Rainer
Kreikemeyer, B.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-04
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Electrical stimulation is a promising approach to enhance bone regeneration while having potential to inhibit bacterial growth.
To investigate effects of alternating electric field stimulation on both human osteoblasts and bacteria, a novel in vitro system was designed.
Electric field distribution was simulated numerically and proved by experimental validation.
Cells were stimulated on Ti6Al4V electrodes and in short distance to electrodes.
Bacterial growth was enumerated in supernatant and on the electrode surface and biofilm formation was quantified.
Electrical stimulation modulated gene expression of osteoblastic differentiation markers in a voltage-dependent manner, resulting in significantly enhanced osteocalcin mRNA synthesis rate on electrodes after stimulation with 1.4VRMS.
While collagen type I synthesis increased when stimulated with 0.2VRMS, it decreased after stimulation with 1.4VRMS.
Only slight and infrequent influence on bacterial growth was observed following stimulations with 0.2VRMS and 1.4VRMS after 48 and 72 h, respectively.
In summary this novel test system is applicable for extended in vitro studies concerning definition of appropriate stimulation parameters for bone cell growth and differentiation, bacterial growth suppression, and investigation of general effects of electrical stimulation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Dauben, Thomas Josef& Ziebart, Josefin& Bender, Thomas& Zaatreh, Sarah& Kreikemeyer, B.& Bader, Rainer. 2016. A Novel In Vitro System for Comparative Analyses of Bone Cells and Bacteria under Electrical Stimulation. BioMed Research International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1098020
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Dauben, Thomas Josef…[et al.]. A Novel In Vitro System for Comparative Analyses of Bone Cells and Bacteria under Electrical Stimulation. BioMed Research International No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1098020
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Dauben, Thomas Josef& Ziebart, Josefin& Bender, Thomas& Zaatreh, Sarah& Kreikemeyer, B.& Bader, Rainer. A Novel In Vitro System for Comparative Analyses of Bone Cells and Bacteria under Electrical Stimulation. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1098020
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1098020
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر