Association between TLR4 and PTEN Involved in LPS-TLR4 Signaling Response
المؤلفون المشاركون
Jiang, Jianxin
Yin, Huahua
Tan, Yan
Wu, Xiaofeng
Yan, Hong
Liu, Feng
Yao, Yuanzhang
Wan, Qi
Li, Lei
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-08-02
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
In this study, we explored the potential mechanisms of how PTEN regulating LPS induced TLR4 signaling pathway.
The initial findings from ELISA demonstrate that PTEN influences TNF-α secretion by its lipid phosphatase activity.
Subsequently, western blot, immunoprecipitation assay, and immunofluorescence were performed to explore the activation process of PTEN by stimulation with LPS.
As early as 20 minutes after LPS stimulation, reduced phosphorylation of PTEN was found obviously.
Accordingly, the whole cell-scattered PTEN translocated towards the cell membrane 20 minutes after stimulating with LPS.
Moreover, the weak physical association between PTEN and TLR4 in resting RAW264.7 cells increased gradually after the stimulation of LPS.
Furthermore, our study showed PTEN decreased LPS-induced Akt activity and upregulated NF- κ B-dependent gene transcription, identifying indirectly that the PTEN could regulate the activation of NF- κ B by its downstream Akt kinase.
In summary, our study illustrates the potential signal transduction process of PTEN while stimulated by LPS: by increasing the association of TLR4, PTEN recruits to its phosphoinositide substrate PI(3,4,5)P3 located on the cell membrane and exerts its dephosphorylated function and subsequently depresses the activity of downstream molecule Akt and results in activation of NF- κ B, followed by the secretion of inflammatory mediators TNF-α.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yin, Huahua& Tan, Yan& Wu, Xiaofeng& Yan, Hong& Liu, Feng& Yao, Yuanzhang…[et al.]. 2016. Association between TLR4 and PTEN Involved in LPS-TLR4 Signaling Response. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098371
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yin, Huahua…[et al.]. Association between TLR4 and PTEN Involved in LPS-TLR4 Signaling Response. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1098371
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yin, Huahua& Tan, Yan& Wu, Xiaofeng& Yan, Hong& Liu, Feng& Yao, Yuanzhang…[et al.]. Association between TLR4 and PTEN Involved in LPS-TLR4 Signaling Response. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098371
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1098371
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر