TNF-α Autocrine Feedback Loops in Human Monocytes: The Pro- and Anti-Inflammatory Roles of the TNF-α Receptors Support the Concept of Selective TNFR1 Blockade In Vivo
المؤلفون المشاركون
Gane, Jennie M.
Stockley, Robert A.
Sapey, Elizabeth
المصدر
Journal of Immunology Research
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-22
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Selective TNFR1 blockade in inflammatory diseases is emerging as a clinical strategy.
We studied the roles of the two TNF-α receptors, TNFR1 and TNFR2, in human monocytes, the principal producer of TNF-α and central to many TNF-α driven diseases.
We hypothesised that TNF-α has pro- and anti-inflammatory effects on monocytes, occurring differentially via TNFR1 and TNFR2.
Monocytes were isolated from healthy human subjects and exposed to LPS, plus/minus the addition of blocking antibodies to TNF-α or its receptors.
Pro- and anti-inflammatory cytokine production was quantified using real-time PCR and ELISAs.
Cell surface expression of TNFR1/2 was measured by flow cytometry.
We demonstrated that monocytes vary in the expression patterns of TNFR1 and TNFR2.
Autocrine binding of TNF-α led to sustained upregulation of proinflammatory cytokines via TNFR1.
In contrast, autocrine binding via TNFR2 upregulated the anti-inflammatory cytokine, IL-10, without proinflammatory effect.
TNFR2 was responsible for binding soluble TNF-α secreted by monocytes, clearing the cytokine from the pericellular environment.
TNFR1 blockade did not change the cell surface expression of TNFR2, leaving this receptor free to upregulate IL-10.
These novel results support the concept of selective TNFR1 blockade in vivo in order that positive anti-inflammatory effects of TNF-α can be retained via TNFR2 ligation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gane, Jennie M.& Stockley, Robert A.& Sapey, Elizabeth. 2016. TNF-α Autocrine Feedback Loops in Human Monocytes: The Pro- and Anti-Inflammatory Roles of the TNF-α Receptors Support the Concept of Selective TNFR1 Blockade In Vivo. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1108702
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gane, Jennie M.…[et al.]. TNF-α Autocrine Feedback Loops in Human Monocytes: The Pro- and Anti-Inflammatory Roles of the TNF-α Receptors Support the Concept of Selective TNFR1 Blockade In Vivo. Journal of Immunology Research No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1108702
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gane, Jennie M.& Stockley, Robert A.& Sapey, Elizabeth. TNF-α Autocrine Feedback Loops in Human Monocytes: The Pro- and Anti-Inflammatory Roles of the TNF-α Receptors Support the Concept of Selective TNFR1 Blockade In Vivo. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1108702
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1108702
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر