Scaffolded Antigens in Yeast Cell Particle Vaccines Provide Protection against Systemic Polyoma Virus Infection
المؤلفون المشاركون
Tipper, Donald J.
Szomolanyi-Tsuda, Eva
المصدر
Journal of Immunology Research
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-04-26
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Background.
U65, a self-aggregating peptide scaffold, traps fused protein antigens in yeast cells.
Conversion to Yeast Cell Particle (YCP) vaccines by partial removal of surface mannoproteins exposes β-glucan, mediating efficient uptake by antigen-presenting cells (APCs).
YCP vaccines are inexpensive, capable of rapid large-scale production and have potential for both parenteral and oral use.
Results.
YCP processing by alkaline hydrolysis exposes up to 20% of the glucan but converts scaffolded antigen and internal yeast proteins into a common aggregate, preventing selective yeast protein removal.
For U65-green fluorescent protein (GFP) or U65-Apolipoprotein A1 (ApoA1) subcutaneous vaccines, maximal IgG responses in mice required 10% glucan exposure.
IgG responses to yeast proteins were 5-fold lower.
Proteolytic mannoprotein removal produced YCPs with only 6% glucan exposure, insufficiently porous for selective removal of even native yeast proteins.
Vaccine efficacy was reduced 10-fold.
Current YCP formulations, therefore, are not suitable for human use but have considerable potential for use in feed animal vaccines.
Significantly, a YCP vaccine expressing a GFP fusion to VP1, the murine polyoma virus major capsid protein, after either oral or subcutaneous administration, protected mice against an intraperitoneal polyoma virus challenge, reducing viral DNA levels in spleen and liver by >98%.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tipper, Donald J.& Szomolanyi-Tsuda, Eva. 2016. Scaffolded Antigens in Yeast Cell Particle Vaccines Provide Protection against Systemic Polyoma Virus Infection. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1108742
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tipper, Donald J.& Szomolanyi-Tsuda, Eva. Scaffolded Antigens in Yeast Cell Particle Vaccines Provide Protection against Systemic Polyoma Virus Infection. Journal of Immunology Research No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1108742
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tipper, Donald J.& Szomolanyi-Tsuda, Eva. Scaffolded Antigens in Yeast Cell Particle Vaccines Provide Protection against Systemic Polyoma Virus Infection. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1108742
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1108742
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر