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PGC-1α-Dependent Mitochondrial Adaptation Is Necessary to Sustain IL-2-Induced Activities in Human NK Cells
المؤلفون المشاركون
Montoya, Margarita
Acuña-Castillo, Claudio
Miranda, Dante
Jara, Claudia
Ibañez, Jorge
Ahumada, Viviana
Martin, Adrian
Córdova, Alexandra
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-20
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Human Natural Killer (NK) cells are a specialized heterogeneous subpopulation of lymphocytes involved in antitumor defense reactions.
NK cell effector functions are critically dependent on cytokines and metabolic activity.
Among various cytokines modulating NK cell function, interleukin-2 (IL-2) can induce a more potent cytotoxic activity defined as lymphokine activated killer activity (LAK).
Our aim was to determine if IL-2 induces changes at the mitochondrial level in NK cells to support the bioenergetic demand for performing this enhanced cytotoxic activity more efficiently.
Purified human NK cells were cultured with high IL-2 concentrations to develop LAK activity, which was assessed by the ability of NK cells to lyse NK-resistant Daudi cells.
Here we show that, after 72 h of culture of purified human NK cells with enough IL-2 to induce LAK activity, both the mitochondrial mass and the mitochondrial membrane potential increased in a PGC-1α-dependent manner.
In addition, oligomycin, an inhibitor of ATP synthase, inhibited IL-2-induced LAK activity at 48 and 72 h of culture.
Moreover, the secretion of IFN-γ from NK cells with LAK activity was also partially dependent on PGC-1α expression.
These results indicate that PGC-1α plays a crucial role in regulating mitochondrial function involved in the maintenance of LAK activity in human NK cells stimulated with IL-2.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Miranda, Dante& Jara, Claudia& Ibañez, Jorge& Ahumada, Viviana& Acuña-Castillo, Claudio& Martin, Adrian…[et al.]. 2016. PGC-1α-Dependent Mitochondrial Adaptation Is Necessary to Sustain IL-2-Induced Activities in Human NK Cells. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1111318
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Miranda, Dante…[et al.]. PGC-1α-Dependent Mitochondrial Adaptation Is Necessary to Sustain IL-2-Induced Activities in Human NK Cells. Mediators of Inflammation No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1111318
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Miranda, Dante& Jara, Claudia& Ibañez, Jorge& Ahumada, Viviana& Acuña-Castillo, Claudio& Martin, Adrian…[et al.]. PGC-1α-Dependent Mitochondrial Adaptation Is Necessary to Sustain IL-2-Induced Activities in Human NK Cells. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1111318
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1111318
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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