Functional Interactions between BKCa α-Subunit and Annexin A5: Implications in Apoptosis
المؤلفون المشاركون
Brazier, Stephen P.
Telezhkin, Vsevolod
Kemp, Paul J.
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-09-25
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Proteomic studies have suggested a biochemical interaction between α subunit of the large conductance, voltage- and Ca2+-activated potassium channel ( B K C a α), and annexin A5 (ANXA5), which we verify here by coimmunoprecipitation and double labelling immunocytochemistry.
The observation that annexin is flipped to the outer membrane leaflet of the plasma membrane during apoptosis, together with the knowledge that the intracellular C-terminal of B K C a α contains both Ca2+-binding and a putative annexin-binding motif, prompted us to investigate the functional consequences of this protein partnership to cell death.
Membrane biotinylation demonstrated that ANXA5 was flipped to the outer membrane leaflet of HEK 293 cells early in serum deprivation-evoked apoptosis.
As expected, serum deprivation caused caspase-3/7 activation and this was accentuated in B K C a α expressing HEK 293 cells.
The functional consequences of ANXA5 partnership with B K C a α were striking, with ANXA5 knockdown causing an increase and ANXA5 overexpression causing a decrease, in single B K C a channel Ca2+-sensitivity, measured in inside-out membrane patches by patch-clamp.
Taken together, these data suggest a novel model of the early stages of apoptosis where membrane flippage results in removal of the inhibitory effect of ANXA5 on K+ channel activity with the consequent amplification of Ca2+ influx and augmented activation of caspases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Brazier, Stephen P.& Telezhkin, Vsevolod& Kemp, Paul J.. 2016. Functional Interactions between BKCa α-Subunit and Annexin A5: Implications in Apoptosis. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1113634
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Brazier, Stephen P.…[et al.]. Functional Interactions between BKCa α-Subunit and Annexin A5: Implications in Apoptosis. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1113634
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Brazier, Stephen P.& Telezhkin, Vsevolod& Kemp, Paul J.. Functional Interactions between BKCa α-Subunit and Annexin A5: Implications in Apoptosis. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1113634
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1113634
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر