A New Method to Simultaneously Quantify the Antioxidants: Carotenes, Xanthophylls, and Vitamin A in Human Plasma
المؤلفون المشاركون
Estruch, Ramón
Lamuela-Raventós, Rosa M.
Colmán-Martínez, Mariel
Martínez-Huélamo, Miriam
Miralles, Esther
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-11-30
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
A simple and accurate reversed phase high-performance liquid chromatography coupled with diode array detector (HPLC-DAD) method for simultaneously determining and quantifying the antioxidants carotenes, xanthophylls, and retinol in human plasma is presented in this paper.
Compounds were extracted with hexane, a C30 column, and a mobile phase of methanol, methyl tert-butyl ether, and water were used for the separation of the compounds.
A total of 8 carotenoids, 3 Z- β -carotene isomers, and 1 fat-soluble vitamin (retinol) were resolved within 72 min at a flow rate of 0.6 mL/min.
Detection was achieved at 450 nm for carotenoids and 330 nm for retinol.
To evaluate the effectiveness of themethod, it has been applied to an intervention study conducted on eight volunteers.
Results.
Limits of detection were between 0.1 μ g/mL for lycopene and astaxanthin and 1.3 μ g/mL for 15-Z- β -carotene.
Recoveries were ranged between 89% and 113% for α -carotene and astaxanthin, respectively.
Accuracy was between 90.7% and 112.2% and precision was between 1% and 15% RSD.
In human plasma samples compounds studied were identified besides three lycopene isomers, demonstrated to be suitable for application in dietary intervention studies.
Conclusions.
Due to its accuracy, precision, selectivity, and reproducibility, this method is suitable to dietary habits and/or antioxidants status studies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Colmán-Martínez, Mariel& Martínez-Huélamo, Miriam& Miralles, Esther& Estruch, Ramón& Lamuela-Raventós, Rosa M.. 2015. A New Method to Simultaneously Quantify the Antioxidants: Carotenes, Xanthophylls, and Vitamin A in Human Plasma. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114740
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Colmán-Martínez, Mariel…[et al.]. A New Method to Simultaneously Quantify the Antioxidants: Carotenes, Xanthophylls, and Vitamin A in Human Plasma. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1114740
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Colmán-Martínez, Mariel& Martínez-Huélamo, Miriam& Miralles, Esther& Estruch, Ramón& Lamuela-Raventós, Rosa M.. A New Method to Simultaneously Quantify the Antioxidants: Carotenes, Xanthophylls, and Vitamin A in Human Plasma. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114740
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1114740
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر