Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells
المؤلفون المشاركون
Zhao, Jing
Mitrofan, Claudia-Gabriela
Appleby, Sarah L.
Morrell, Nicholas W.
Lever, Andrew M. L.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-16
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
Late outgrowth endothelial progenitor cells (LO-EPC) possess a high proliferative potential, differentiate into vascular endothelial cells (EC), and form networks, suggesting they play a role in vascular repair.
However, due to their scarcity in the circulation there is a requirement for ex vivo expansion before they could provide a practical cell therapy and it is currently unclear if they would home and engraft to an injury site.
Using an in vitro flow system we studied LO-EPC under simulated injury conditions including EC activation, ischaemia, disrupted EC integrity, and exposed basement membrane.
Perfused LO-EPC adhered to discontinuous EC paracellularly at junctional regions between adjacent cells under shear stress 0.7 dyn/cm2.
The interaction was not adhesion molecule-dependent and not enhanced by EC activation.
LO-EPC expressed high levels of the VE-Cadherin which may explain these findings.
Ischaemia reperfusion injury decreased the interaction with LO-EPC due to cell retraction.
LO-EPC interacted with exposed extracellular matrix (ECM) proteins, fibronectin and vitronectin.
The interaction was mediated by integrins α5β3, αvβ1, and αvβ3.
This study has demonstrated that an injured local environment presents sufficient adhesive signals to capture flow perfused LO-EPC in vitro and that LO-EPC have properties consistent with their potential role in vascular repair.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhao, Jing& Mitrofan, Claudia-Gabriela& Appleby, Sarah L.& Morrell, Nicholas W.& Lever, Andrew M. L.. 2016. Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116035
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhao, Jing…[et al.]. Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-13.
https://search.emarefa.net/detail/BIM-1116035
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhao, Jing& Mitrofan, Claudia-Gabriela& Appleby, Sarah L.& Morrell, Nicholas W.& Lever, Andrew M. L.. Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1116035
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116035
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر