The Interaction between Adult Cardiac Fibroblasts and Embryonic Stem Cell-Derived Cardiomyocytes Leads to Proarrhythmic Changes in In Vitro Cocultures
المؤلفون المشاركون
Trieschmann, Jan
Bettin, Daniel
Haustein, Moritz
Köster, Annette
Molcanyi, Marek
Halbach, Marcel
Hanna, Mira
Fouad, Mariam
Brockmeier, Konrad
Hescheler, Jürgen
Pfannkuche, Kurt
Hannes, Tobias
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-01-05
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Transplantation of stem cell-derived cardiomyocytes is one of the most promising therapeutic approaches after myocardial infarction, as loss of cardiomyocytes is virtually irreversible by endogenous repair mechanisms.
In myocardial scars, transplanted cardiomyocytes will be in immediate contact with cardiac fibroblasts.
While it is well documented how the electrophysiology of neonatal cardiomyocytes is modulated by cardiac fibroblasts of the same developmental stage, it is unknown how adult cardiac fibroblasts (aCFs) affect the function of embryonic stem cell-derived cardiomyocytes (ESC-CMs).
To investigate the effects of aCFs on ESC-CM electrophysiology, we performed extra- and intracellular recordings of murine aCF-ESC-CM cocultures.
We observed that spontaneous beating behaviour was highly irregular in aCF-ESC-CM cocultures compared to cocultures with mesenchymal stem cells (coefficient of variation of the interspike interval: 40.5±15.2% versus 9.3±2.0%, p=0.008) and that action potential amplitude and maximal upstroke velocity (Vmax) were reduced (amplitude: 52.3±1.7 mV versus 65.1±1.5 mV, Vmax: 7.0±1.0 V/s versus 36.5±5.3 V/s), while action potential duration (APD) was prolonged (APD50: 25.6±1.0 ms versus 16.8±1.9 ms, p<0.001; APD90: 52.2±1.5 ms versus 43.3±3.3 ms, p<0.01) compared to controls.
Similar changes could be induced by aCF-conditioned medium.
We conclude that the presence of aCFs changes automaticity and induces potentially proarrhythmic changes of ESC-CM electrophysiology.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Trieschmann, Jan& Bettin, Daniel& Haustein, Moritz& Köster, Annette& Molcanyi, Marek& Halbach, Marcel…[et al.]. 2016. The Interaction between Adult Cardiac Fibroblasts and Embryonic Stem Cell-Derived Cardiomyocytes Leads to Proarrhythmic Changes in In Vitro Cocultures. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116319
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Trieschmann, Jan…[et al.]. The Interaction between Adult Cardiac Fibroblasts and Embryonic Stem Cell-Derived Cardiomyocytes Leads to Proarrhythmic Changes in In Vitro Cocultures. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116319
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Trieschmann, Jan& Bettin, Daniel& Haustein, Moritz& Köster, Annette& Molcanyi, Marek& Halbach, Marcel…[et al.]. The Interaction between Adult Cardiac Fibroblasts and Embryonic Stem Cell-Derived Cardiomyocytes Leads to Proarrhythmic Changes in In Vitro Cocultures. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116319
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116319
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر