Human Umbilical Cord Blood-Derived Serum for Culturing the Supportive Feeder Cells of Human Pluripotent Stem Cell Lines
المؤلفون المشاركون
Rungsiwiwut, Ruttachuk
Ingrungruanglert, Praewphan
Numchaisrika, Pranee
Virutamasen, Pramuan
Phermthai, Tatsanee
Pruksananonda, Kamthorn
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-12-29
دولة النشر
مصر
عدد الصفحات
12
الملخص EN
Although human pluripotent stem cells (hPSCs) can proliferate robustly on the feeder-free culture system, genetic instability of hPSCs has been reported in such environment.
Alternatively, feeder cells enable hPSCs to maintain their pluripotency.
The feeder cells are usually grown in a culture medium containing fetal bovine serum (FBS) prior to coculture with hPSCs.
The use of FBS might limit the clinical application of hPSCs.
Recently, human cord blood-derived serum (hUCS) showed a positive effect on culture of mesenchymal stem cells.
It is interesting to test whether hUCS can be used for culture of feeder cells of hPSCs.
This study was aimed to replace FBS with hUCS for culturing the human foreskin fibroblasts (HFFs) prior to feeder cell preparation.
The results showed that HFFs cultured in hUCS-containing medium (HFF-hUCS) displayed fibroblastic features, high proliferation rates, short population doubling times, and normal karyotypes after prolonged culture.
Inactivated HFF-hUCS expressed important genes, including Activin A, FGF2, and TGFβ1, which have been implicated in the maintenance of hPSC pluripotency.
Moreover, hPSC lines maintained pluripotency, differentiation capacities, and karyotypic stability after being cocultured for extended period with inactivated HFF-hUCS.
Therefore, the results demonstrated the benefit of hUCS for hPSCs culture system.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Rungsiwiwut, Ruttachuk& Ingrungruanglert, Praewphan& Numchaisrika, Pranee& Virutamasen, Pramuan& Phermthai, Tatsanee& Pruksananonda, Kamthorn. 2015. Human Umbilical Cord Blood-Derived Serum for Culturing the Supportive Feeder Cells of Human Pluripotent Stem Cell Lines. Stem Cells International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116594
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Rungsiwiwut, Ruttachuk…[et al.]. Human Umbilical Cord Blood-Derived Serum for Culturing the Supportive Feeder Cells of Human Pluripotent Stem Cell Lines. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-12.
https://search.emarefa.net/detail/BIM-1116594
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Rungsiwiwut, Ruttachuk& Ingrungruanglert, Praewphan& Numchaisrika, Pranee& Virutamasen, Pramuan& Phermthai, Tatsanee& Pruksananonda, Kamthorn. Human Umbilical Cord Blood-Derived Serum for Culturing the Supportive Feeder Cells of Human Pluripotent Stem Cell Lines. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1116594
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116594
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر