Inhibition of JAK-STAT ERKMAPK and Glycogen Synthase Kinase-3 Induces a Change in Gene Expression Profile of Bovine Induced Pluripotent Stem Cells
المؤلفون المشاركون
Verma, Paul J.
Malaver-Ortega, Luis F.
Sumer, Huseyin
Liu, Jun
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-01-06
دولة النشر
مصر
عدد الصفحات
11
الملخص EN
Pluripotent stem cells (PSCs) fall in two states, one highly undifferentiated, the naïve state, and the primed state, characterized by the inability to contribute to germinal lineage.
Several reports have demonstrated that these states can be modified by changes to the cell culture conditions.
With the advent of nuclear reprogramming, bovine induced pluripotent stem cells (biPSCs) have been generated.
These cells represent examples of a transient-intermediate state of pluripotency with remarkable characteristics and biotechnological potential.
Herein, we generated and characterized biPSC.
Next, we evaluated different culture conditions for the ability to affect the expression of the set of core pluripotent transcription factors in biPSC.
It was found that the use of 6-bromoindirubin-3-oxime and Sc1 inhibitors alone or in combination with 5-AzaC induced significantly higher levels of expression of endogenous REX1, OCT4, NANOG, and SOX2.
Furthermore, LIF increased the levels of expression of OCT4 and REX1, compared with those cultured with LIF + bFGF.
By contrast, bFGF decreased the levels of expression for both REX1 and OCT4.
These results demonstrate that the biPSC gene expression profile is malleable by modification of the cell culture conditions well after nuclear reprogramming, and the culture conditions may determine their differentiation potential.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Malaver-Ortega, Luis F.& Sumer, Huseyin& Liu, Jun& Verma, Paul J.. 2016. Inhibition of JAK-STAT ERKMAPK and Glycogen Synthase Kinase-3 Induces a Change in Gene Expression Profile of Bovine Induced Pluripotent Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1116703
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Malaver-Ortega, Luis F.…[et al.]. Inhibition of JAK-STAT ERKMAPK and Glycogen Synthase Kinase-3 Induces a Change in Gene Expression Profile of Bovine Induced Pluripotent Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1116703
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Malaver-Ortega, Luis F.& Sumer, Huseyin& Liu, Jun& Verma, Paul J.. Inhibition of JAK-STAT ERKMAPK and Glycogen Synthase Kinase-3 Induces a Change in Gene Expression Profile of Bovine Induced Pluripotent Stem Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1116703
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1116703
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر