α -Hederin Arrests Cell Cycle at G2M Checkpoint and Promotes Mitochondrial Apoptosis by Blocking Nuclear Factor-κB Signaling in Colon Cancer Cells
المؤلفون المشاركون
Yang, Ye
Cheng, Hai-Bo
Sun, Dongdong
Shen, Weixing
Zhang, Feng
Fan, Huisen
Tan, Jiani
Li, Liu
Xu, Changliang
Zhang, Haibin
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-09-27
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Colon cancer represents the third most common malignancy worldwide.
New drugs with high efficaciousness and safety for the treatment of colon cancer are urgently needed in clinical context.
Here, we were aimed to evaluate the antitumor activity of the natural compound α-hederin in human colon cancer cells.
We treated SW620 cells with interleukin-6 (IL-6) in vitro to mimic the paracrine inflammatory microenvironment of tumor cells.
α-Hederin concentration dependently reduced the viability of IL-6-stimulated SW620 cells.
α-Hederin increased the number of IL-6-stimulated SW620 cells at the G2/M phase and reduced the mRNA and protein expression of cyclin B1 and CDK1.
Moreover, α-hederin induced apoptosis and loss of mitochondrial membrane potential in IL-6-stimulated SW620 cells.
α-Hederin downregulated Bcl-2 expression, upregulated Bax expression, and promoted cytochrome c release from mitochondria into cytoplasm.
Additionally, α-hederin elevated the levels of cleaved-caspase-9, cleaved-caspase-3, and cleaved-PARP, but had little effects on the levels of cleaved-caspase-8.
Moreover, α-hederin prevented the nuclear translocation of nuclear factor-κB (NF-κB) and reduced the phosphorylation of IκBα and IKKα, suggesting the blockade of NF-κB signaling.
NF-κB inhibitor PDTC not only produced similar proapoptotic effects on IL-6-stimulated SW620 cells as α-hederin did, but also synergistically enhanced α-hederin’s proapoptotic effects.
Furthermore, α-hederin inhibited the phosphorylation of ERK in IL-6-stimulated SW620 cells, which was involved in α-hederin blockade of NF-κB nuclear translocation.
Altogether, α-hederin suppressed viability, induced G2/M cell cycle arrest, and stimulated mitochondrial and caspase-dependent apoptosis in colon cancer cells, which were associated with disruption of NF-κB and ERK pathways, suggesting α-hederin as a promising candidate for intervention of colon cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Sun, Dongdong& Shen, Weixing& Zhang, Feng& Fan, Huisen& Tan, Jiani& Li, Liu…[et al.]. 2018. α -Hederin Arrests Cell Cycle at G2M Checkpoint and Promotes Mitochondrial Apoptosis by Blocking Nuclear Factor-κB Signaling in Colon Cancer Cells. BioMed Research International،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1125108
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Sun, Dongdong…[et al.]. α -Hederin Arrests Cell Cycle at G2M Checkpoint and Promotes Mitochondrial Apoptosis by Blocking Nuclear Factor-κB Signaling in Colon Cancer Cells. BioMed Research International No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1125108
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Sun, Dongdong& Shen, Weixing& Zhang, Feng& Fan, Huisen& Tan, Jiani& Li, Liu…[et al.]. α -Hederin Arrests Cell Cycle at G2M Checkpoint and Promotes Mitochondrial Apoptosis by Blocking Nuclear Factor-κB Signaling in Colon Cancer Cells. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1125108
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125108
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر