Molecular Cloning and Characterization of a Novel α-Amylase from Antarctic Sea Ice Bacterium Pseudoalteromonas sp. M175 and Its Primary Application in Detergent
المؤلفون المشاركون
Wang, Xiaofei
Kan, Guangfeng
Ren, Xiulian
Yu, Geng
Shi, Cuijuan
Xie, Qiuju
Wen, Hua
Betenbaugh, Michael
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-06-27
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
A novel cold-adapted and salt-tolerant α-amylase gene (amy175) from Antarctic sea ice bacterium Pseudoalteromonas sp.
M175 was successfully cloned and expressed.
The open reading frame (ORF) of amy175 had 1722 bp encoding a protein of 573 amino acids residues.
Multiple alignments indicated Amy175 had seven highly conserved sequences and the putative catalytic triad (Asp244, Glu286, and Asp372).
It was the first identified member of GH13_36 subfamily which contained QPDLN in the CSR V.
The recombinant enzyme (Amy175) was purified to homogeneity with a molecular mass of about 62 kDa on SDS-PAGE.
It had a mixed enzyme specificity of α-amylase and α-glucosidase.
Amy175 displayed highest activity at pH 8.0 and 25°C and exhibited extreme salt-resistance with the maximum activity at 1 M NaCl.
Amy175 was strongly stimulated by Mg2+, Ni2+, K+, 1 mM Ca2+, 1 mM Ba2+, 1 mM Pb2+, 1 mM sodium dodecyl sulphate (SDS), and 10% dimethyl sulfoxide (DMSO) but was significantly inhibited by Cu2+, Mn2+, Hg2+, 10 mM β-mercaptoethanol (β-ME), and 10% Tween 80.
Amy175 demonstrated excellent resistance towards all the tested commercial detergents, and wash performance analysis displayed that the addition of Amy175 improved the stain removal efficiency.
This study demonstrated that Amy175 would be proposed as a novel α-amylase source for industrial application in the future.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Xiaofei& Kan, Guangfeng& Ren, Xiulian& Yu, Geng& Shi, Cuijuan& Xie, Qiuju…[et al.]. 2018. Molecular Cloning and Characterization of a Novel α-Amylase from Antarctic Sea Ice Bacterium Pseudoalteromonas sp. M175 and Its Primary Application in Detergent. BioMed Research International،Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1125743
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Xiaofei…[et al.]. Molecular Cloning and Characterization of a Novel α-Amylase from Antarctic Sea Ice Bacterium Pseudoalteromonas sp. M175 and Its Primary Application in Detergent. BioMed Research International No. 2018 (2018), pp.1-16.
https://search.emarefa.net/detail/BIM-1125743
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Xiaofei& Kan, Guangfeng& Ren, Xiulian& Yu, Geng& Shi, Cuijuan& Xie, Qiuju…[et al.]. Molecular Cloning and Characterization of a Novel α-Amylase from Antarctic Sea Ice Bacterium Pseudoalteromonas sp. M175 and Its Primary Application in Detergent. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-16.
https://search.emarefa.net/detail/BIM-1125743
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125743
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر