mTORC2 Regulates Lipogenic Gene Expression through PPARγ to Control Lipid Synthesis in Bovine Mammary Epithelial Cells
المؤلفون المشاركون
Guo, Zhixin
Zhao, Keyu
Feng, Xue
Yan, Dandan
Yao, Ruiyuan
Chen, Yuhao
Bao, Lili
Wang, Zhigang
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-05-16
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The mechanistic target of rapamycin complex 2 (mTORC2) primarily functions as an effector of insulin/PI3K signaling to regulate cell proliferation and is associated with cell metabolism.
However, the function of mTORC2 in lipid metabolism is not well understood.
In the present study, mTORC2 was inactivated by the ATP-competitive mTOR inhibitor AZD8055 or shRNA targeting RICTOR in primary bovine mammary epithelial cells (pBMECs).
MTT assay was performed to examine the effect of AZD8055 on cell proliferation.
ELISA assay and GC-MS analysis were used to determine the content of lipid.
The mRNA and protein expression levels were investigated by RT/real-time PCR and western blot analysis, respectively.
We found that cell proliferation, mTORC2 activation, and lipid secretion were inhibited by AZD8055.
RICTOR was knocked down and mTORC2 activation was specifically attenuated by the shRNA.
Compared to control cells, the expression of the transcription factor gene PPARG and the lipogenic genes LPIN1, DGAT1, ACACA, and FASN was downregulated in RICTOR silencing cells.
As a result, the content of intracellular triacylglycerol (TAG), palmitic acid (PA), docosahexaenoic acid (DHA), and other 16 types of fatty acid was decreased in the treated cells; the accumulation of TAG, PA, and DHA in cell culture medium was also reduced.
Overall, mTORC2 plays a critical role in regulating lipogenic gene expression, lipid synthesis, and secretion in pBMECs, and this process probably is through PPARγ.
This finding provides a model by which lipogenesis is regulated in pBMECs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Guo, Zhixin& Zhao, Keyu& Feng, Xue& Yan, Dandan& Yao, Ruiyuan& Chen, Yuhao…[et al.]. 2019. mTORC2 Regulates Lipogenic Gene Expression through PPARγ to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. BioMed Research International،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1125878
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Guo, Zhixin…[et al.]. mTORC2 Regulates Lipogenic Gene Expression through PPARγ to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. BioMed Research International No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1125878
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Guo, Zhixin& Zhao, Keyu& Feng, Xue& Yan, Dandan& Yao, Ruiyuan& Chen, Yuhao…[et al.]. mTORC2 Regulates Lipogenic Gene Expression through PPARγ to Control Lipid Synthesis in Bovine Mammary Epithelial Cells. BioMed Research International. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1125878
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1125878
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر