Hydrogen-Rich Saline Activated Autophagy via HIF-1α Pathways in Neuropathic Pain Model
المؤلفون المشاركون
Xie, Keliang
Wang, Huixing
Huo, Xiaodong
Chen, Hongguang
Li, Bo
Liu, Jingzhi
Ma, Wenting
Wang, Xiaojuan
Yu, Yonghao
Shi, Kemei
المصدر
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-05-17
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Background.
Neuropathic pain is a chronic and intractable pain, with very few effective analgesics.
It involves an impaired cell autophagy process.
Hydrogen-rich saline (HRS) reportedly reduces allodynia and hyperalgesia in a neuropathic pain model; however, it is unknown whether these effects involve autophagy induction.
Methods.
We investigated the relationship between HRS and cell autophagy in a neuropathic pain model generated by chronic constriction injury (CCI) in Sprague–Dawley rats.
Rats received an intraperitoneal injection of HRS (10 mL/kg daily, from 1 day before until 14 days after CCI), 3MA (autophagy inhibitor), 2ME2 (HIF-1α inhibitor), or EDHB (HIF-1α agonist).
The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were tested 1 day before and 1, 3, 7, 10, and 14 days after the operation.
HIF-1α and cell autophagy markers in the spinal cord were evaluated by western blotting and real-time PCR assays at 14 days after CCI.
Autophagosomes with double membranes were identified by transmission electron microscopy.
Results.
CCI caused behavioral hypersensitivity to mechanical and thermal stimulation in the hind-paw of the injured side.
HRS improved MWT and TWL, activated autophagy, and increased autophagosomes and autolysosomes in CCI rats.
3-MA aggravated hyperalgesia and allodynia and suppressed autophagy, while EDHB attenuated hyperalgesia and activated the autophagy procedure and the HIF-1α downstream target gene BNIP3.
HIF-1α inhibitors reversed the regulatory effects of HRS on autophagy in CCI rats at 14 days after spinal cord injury.
Conclusion.
HRS reduced mechanical hyperalgesia and activation of cell autophagy in neuropathic pain through a HIF1-dependent pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Huixing& Huo, Xiaodong& Chen, Hongguang& Li, Bo& Liu, Jingzhi& Ma, Wenting…[et al.]. 2018. Hydrogen-Rich Saline Activated Autophagy via HIF-1α Pathways in Neuropathic Pain Model. BioMed Research International،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1126839
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Huixing…[et al.]. Hydrogen-Rich Saline Activated Autophagy via HIF-1α Pathways in Neuropathic Pain Model. BioMed Research International No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1126839
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Huixing& Huo, Xiaodong& Chen, Hongguang& Li, Bo& Liu, Jingzhi& Ma, Wenting…[et al.]. Hydrogen-Rich Saline Activated Autophagy via HIF-1α Pathways in Neuropathic Pain Model. BioMed Research International. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1126839
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1126839
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر