Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line
المؤلفون المشاركون
Ideris, Aini
Hair-Bejo, Mohd
Lawal, Nafi’u
Arshad, Siti Suri
Omar, Abdul Rahman
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-19، 19ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-05
دولة النشر
مصر
عدد الصفحات
19
التخصصات الرئيسية
الملخص EN
Two Malaysian very virulent infectious bursal disease virus (vvIBDV) strains UPM0081 and UPM190 (also known as UPMB00/81 and UPM04/190, respectively) isolated from local IBD outbreaks were serially passaged 12 times (EP12) in specific pathogen free (SPF) chicken embryonated eggs (CEE) by chorioallantoic membrane (CAM) route.
The EP12 isolate was further adapted and serially propagated in BGM-70 cell line up to 20 passages (P20).
Characteristic cytopathic effects (CPEs) were subtly observed at P1 in both isolates 72 hours postinoculation (pi).
The CPE became prominent at P5 with cell rounding, cytoplasmic vacuoles, granulation, and detachment from flask starting from day 3 pi, up to 7 days pi with titers of 109.50 TCID50/mL and log109.80 TCID50/mL for UPM0081 and UPM190, respectively.
The CPE became subtle at P17 and disappeared by P18 and P19 for UPM0081 and UPM190, respectively.
However, the presence of IBDV was confirmed by immunoperoxidase, immunofluorescence, and RT-PCR techniques.
Phylogenetic analysis showed that these two isolates were of the vvIBDV.
It appears that a single mutation of UPM190 and UPM0081 IBDV isolates at D279N could facilitate vvIBDV strain adaptability in CEE and BGM-70 cultures.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lawal, Nafi’u& Hair-Bejo, Mohd& Arshad, Siti Suri& Omar, Abdul Rahman& Ideris, Aini. 2017. Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line. Advances in Virology،Vol. 2017, no. 2017, pp.1-19.
https://search.emarefa.net/detail/BIM-1131097
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lawal, Nafi’u…[et al.]. Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line. Advances in Virology No. 2017 (2017), pp.1-19.
https://search.emarefa.net/detail/BIM-1131097
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lawal, Nafi’u& Hair-Bejo, Mohd& Arshad, Siti Suri& Omar, Abdul Rahman& Ideris, Aini. Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line. Advances in Virology. 2017. Vol. 2017, no. 2017, pp.1-19.
https://search.emarefa.net/detail/BIM-1131097
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1131097
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر