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Metformin’s Effects on Apoptosis of Esophageal Carcinoma Cells and Normal Esophageal Epithelial Cells: An In Vitro Comparative Study
المؤلفون المشاركون
Peng, Jianwei
Jing, Xubin
Wu, Jialing
Hong, Danmian
Hu, Xi
Wang, Qinjia
Hu, Hui
Cai, Xianbin
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-18
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The effect of metformin on human esophageal normal and carcinoma cells remains poorly understood.
We aim to investigate the different antiproliferation effects and underlying distinct molecular mechanisms between these two types of cells.
Human esophageal squamous cell carcinoma cell line, EC109, and normal esophageal epithelial cell line, HEEC, were used in the experiment.
The cell survival rate was determined by cell counting kit-8 (CCK-8).
Cell apoptosis was analyzed by flow cytometry.
The mRNA and protein levels of signal transducer and activator of transcription 3 (Stat3) were detected by real-time quantitative PCR and western blot.
Interleukin-6 (IL-6) was added to activate Stat3.
The level of intracellular reactive oxygen species (ROS) was assessed by a DCFH-DA fluorescent probe.
Metformin had more significant inhibitory effects on cell proliferation in EC109 cells than HEECs.
Metformin induced apoptosis of EC109 cells in a dose-dependent manner instead of HEECs.
The expression of Stat3 in both mRNA and protein levels was higher in EC109 cells than HEECs.
Further study revealed that metformin may attenuate the phosphorylation of the Stat3 and the Bcl-2 expression, which was restored by IL-6 partly in EC109 cells but not HEECs.
On the contrary, metformin increased the level of ROS in both the cell lines, but this intracellular ROS variation had no effect on apoptosis.
Metformin has different functional roles on the apoptosis in esophageal carcinoma cells and normal esophageal cells.
Therefore, the Stat3/Bcl-2 pathway-mediated apoptosis underlies the cell-type-specific drug sensitivity, suggesting metformin possesses a therapeutic activity and selectivity on esophageal cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Peng, Jianwei& Jing, Xubin& Wu, Jialing& Hong, Danmian& Hu, Xi& Wang, Qinjia…[et al.]. 2020. Metformin’s Effects on Apoptosis of Esophageal Carcinoma Cells and Normal Esophageal Epithelial Cells: An In Vitro Comparative Study. BioMed Research International،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1131434
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Peng, Jianwei…[et al.]. Metformin’s Effects on Apoptosis of Esophageal Carcinoma Cells and Normal Esophageal Epithelial Cells: An In Vitro Comparative Study. BioMed Research International No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1131434
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Peng, Jianwei& Jing, Xubin& Wu, Jialing& Hong, Danmian& Hu, Xi& Wang, Qinjia…[et al.]. Metformin’s Effects on Apoptosis of Esophageal Carcinoma Cells and Normal Esophageal Epithelial Cells: An In Vitro Comparative Study. BioMed Research International. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1131434
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1131434
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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