Evaluation of 99mTc-HYNIC-VCAM-1scFv as a Potential Qualitative and Semiquantitative Probe Targeting Various Tumors
المؤلفون المشاركون
Zhang, Yongxue
Lan, Xiaoli
Zhang, Xiao
Hu, Fan
Liu, Chunbao
Yin, Lianglan
Zhang, Yingying
المصدر
Contrast Media & Molecular Imaging
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-05-03
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Vascular cell adhesion molecule 1 (VCAM-1) is overexpressed in varieties of cancers.
This study aimed to evaluate the application of a single chain variable fragment (scFv) of anti-VCAM-1 antibody labeled with 99mTc as a possible imaging agent in several tumors.
VCAM-1 scFv was labeled with 99mTc using succinimidyl 6-hydrazinium nicotinate hydrochloride, and 99mTc-HYNIC-VCAM-1scFv was successfully synthesized with a high radiolabeling yield.
VCAM-1 expression was evaluated in six cell lines by immunofluorescence staining.
In vitro binding assays showed different binding affinities of 99mTc-HYNIC-VCAM-1scFv in different tumor cell lines, with high uptake in B16F10 melanoma and HT1080 fibrosarcoma cells, which was consistent with immunofluorescence staining results.
In vivo SPECT planar imaging demonstrated that B16F10 and HT1080 tumors could be clearly visualized.
Less intense uptake was observed in human SKOV3.ip ovarian tumor, and weak uptake was observed in human A375m melanoma, MDA-MB-231 breast cancer, and 786-O renal tumors.
These findings were confirmed by biodistribution and immunofluorescence studies.
High uptake by B16F10 tumors was inhibited by excess unlabeled VCAM-1scFv.
99mTc-HYNIC-VCAM-1scFv, which selectively binds to VCAM-1, can provide a qualitative and semiquantitative method for noninvasive evaluation of VCAM-1 expression by tumors.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Xiao& Hu, Fan& Liu, Chunbao& Yin, Lianglan& Zhang, Yingying& Zhang, Yongxue…[et al.]. 2018. Evaluation of 99mTc-HYNIC-VCAM-1scFv as a Potential Qualitative and Semiquantitative Probe Targeting Various Tumors. Contrast Media & Molecular Imaging،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1131539
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Xiao…[et al.]. Evaluation of 99mTc-HYNIC-VCAM-1scFv as a Potential Qualitative and Semiquantitative Probe Targeting Various Tumors. Contrast Media & Molecular Imaging No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1131539
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Xiao& Hu, Fan& Liu, Chunbao& Yin, Lianglan& Zhang, Yingying& Zhang, Yongxue…[et al.]. Evaluation of 99mTc-HYNIC-VCAM-1scFv as a Potential Qualitative and Semiquantitative Probe Targeting Various Tumors. Contrast Media & Molecular Imaging. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1131539
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1131539
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر