Cloning, Expression, and Immunogenicity of Fimbrial-F17A Subunit Vaccine against Escherichia coli Isolated from Bovine Mastitis
المؤلفون المشاركون
Han, Bo
Ali, Tariq
Gao, Jian
Chen, Wei
Liu, Yongxia
Yin, Jinhua
Deng, Youtian
Zhang, Ju
Cheng, Jia
ur Rahman, Sadeeq
المصدر
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-11-29
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
There is a need to identify and select new promising immunodominant antigens that have the ability to provide protective immunity against E.
coli causing bovine mastitis.
Recently we showed that f17a was found to be the most prevalent and crucial virulent factor among the pathogenic E.
coli isolated from bovine mastitis.
Here, in this report, the recombinant F17A based subunit vaccine adjuvant with MF59 was tested for immunogenicity against E.
coli in a murine model.
The vaccinated mice did not show any abnormal behavioral changes and histopathological lesions after vaccination.
The specific antibody level against F17A was significantly higher in MF59-adjuvant-group, and also lasted for longer duration with a significant (P<0.01) production level of IgG1 and IgG2a.
Moreover, we noted higher survival rate in mice injected with F17A-MF59-adjuvant group after challenging with the clinical E.
coli strain.
Our findings of bacterial clearance test revealed that elimination rate from liver, spleen, and kidney in MF59-adjuvant-group was significantly higher than the control group.
Finally, the proportion of CD4+T cells was increased, while CD8+ was decreased in MF59-adjuvant group.
In conclusion, the current study reveals the capability of F17A-MF59 as a potential vaccine candidate against pathogenic E.
coli causing mastitis in dairy animals.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Wei& Liu, Yongxia& Yin, Jinhua& Deng, Youtian& Ali, Tariq& Zhang, Ju…[et al.]. 2017. Cloning, Expression, and Immunogenicity of Fimbrial-F17A Subunit Vaccine against Escherichia coli Isolated from Bovine Mastitis. BioMed Research International،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1135865
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Wei…[et al.]. Cloning, Expression, and Immunogenicity of Fimbrial-F17A Subunit Vaccine against Escherichia coli Isolated from Bovine Mastitis. BioMed Research International No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1135865
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Wei& Liu, Yongxia& Yin, Jinhua& Deng, Youtian& Ali, Tariq& Zhang, Ju…[et al.]. Cloning, Expression, and Immunogenicity of Fimbrial-F17A Subunit Vaccine against Escherichia coli Isolated from Bovine Mastitis. BioMed Research International. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1135865
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1135865
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر