Nimbolide Represses the Proliferation, Migration, and Invasion of Bladder Carcinoma Cells via Chk2-Mediated G2M Phase Cell Cycle Arrest, Altered Signaling Pathways, and Reduced Transcription Factors-Associated MMP-9 Expression
المؤلفون المشاركون
Kim, Wun-Jae
Kim, Gonhyung
Shin, Seung-Shick
Hwang, Byungdoo
Muhammad, Kashif
Gho, Yujeong
Song, Jun-Hui
Moon, Sung-Kwon
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-07-14
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Nimbolide, an active chemical constituent of Azadirachta indica, reportedly has several physiological effects.
Here, we assessed novel anticancer effects of nimbolide against bladder cancer EJ and 5637 cells.
Nimbolide treatment inhibited the proliferation of both bladder cancer cell lines with an IC50 value of 3 μM.
Treatment of cells with nimbolide induced G2/M phase cell cycle arrest via both Chk2-Cdc25C-Cdc2/cyclin B1-Wee1 pathway and Chk2-p21WAF1-Cdc2/cyclin B1-Wee1 pathway.
Nimbolide increased JNK phosphorylation and decreased p38MAPK and AKT phosphorylation.
Additionally, nimbolide impeded both wound healing migration and invasion abilities by suppressing matrix metalloproteinase-9 (MMP-9) activity.
Finally, nimbolide repressed the binding activity of NF-κB, Sp-1, and AP-1 motifs, which are key transcription factors for MMP-9 activity regulation.
Overall, our study indicates that nimbolide is a potential chemotherapeutic agent for bladder cancer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shin, Seung-Shick& Hwang, Byungdoo& Muhammad, Kashif& Gho, Yujeong& Song, Jun-Hui& Kim, Wun-Jae…[et al.]. 2019. Nimbolide Represses the Proliferation, Migration, and Invasion of Bladder Carcinoma Cells via Chk2-Mediated G2M Phase Cell Cycle Arrest, Altered Signaling Pathways, and Reduced Transcription Factors-Associated MMP-9 Expression. Evidence-Based Complementary and Alternative Medicine،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1149568
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shin, Seung-Shick…[et al.]. Nimbolide Represses the Proliferation, Migration, and Invasion of Bladder Carcinoma Cells via Chk2-Mediated G2M Phase Cell Cycle Arrest, Altered Signaling Pathways, and Reduced Transcription Factors-Associated MMP-9 Expression. Evidence-Based Complementary and Alternative Medicine No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1149568
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shin, Seung-Shick& Hwang, Byungdoo& Muhammad, Kashif& Gho, Yujeong& Song, Jun-Hui& Kim, Wun-Jae…[et al.]. Nimbolide Represses the Proliferation, Migration, and Invasion of Bladder Carcinoma Cells via Chk2-Mediated G2M Phase Cell Cycle Arrest, Altered Signaling Pathways, and Reduced Transcription Factors-Associated MMP-9 Expression. Evidence-Based Complementary and Alternative Medicine. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1149568
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1149568
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر