EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs

المؤلفون المشاركون

Lim, Sai Kiang
Mandorfer, Mattias
Reiner, Agnes T.
Tan, Sisareuth
Agreiter, Christiane
Auer, Katharina
Bachmayr-Heyda, Anna
Aust, Stefanie
Pecha, Nina
Pils, Dietmar
Brisson, Alain R.
Zeillinger, Robert

المصدر

Disease Markers

العدد

المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2017-05-18

دولة النشر

مصر

عدد الصفحات

9

التخصصات الرئيسية

الأمراض

الملخص EN

High-grade serous ovarian cancer (HGSOC) is the most aggressive type of ovarian cancer and is responsible for most deaths caused by gynecological cancers.

Numerous candidate biomarkers were identified for this disease in the last decades, but most were not sensitive or specific enough for clinical applications.

Hence, new biomarkers for HGSOC are urgently required.

This study aimed to identify new markers by isolating different extracellular vesicle (EV) types from the ascites of ovarian cancer patients according to their affinities for lipid-binding proteins and analyzing their protein cargo.

This approach circumvents the low signal-to-noise ratio when using biological fluids for biomarker discovery and the issue of contamination by large non-EV complexes.

We isolated and analyzed three distinct EV populations from the ascites of patients with ovarian cancer or cirrhosis and observed that Annexin V-binding EVs have higher levels of matrix metalloproteinase 9 in malignant compared to portal-hypertensive ascites.

As this protein was not detected in other EV populations, this study validates our approach of using different EV types for optimal biomarker discovery.

Furthermore, MMP9 in Annexin V-binding EVs could be a HGSOC biomarker with enhanced specificity, because its identification requires detection of two distinct components, that is, lipid and protein.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Reiner, Agnes T.& Tan, Sisareuth& Agreiter, Christiane& Auer, Katharina& Bachmayr-Heyda, Anna& Aust, Stefanie…[et al.]. 2017. EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs. Disease Markers،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1152624

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Reiner, Agnes T.…[et al.]. EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs. Disease Markers No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1152624

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Reiner, Agnes T.& Tan, Sisareuth& Agreiter, Christiane& Auer, Katharina& Bachmayr-Heyda, Anna& Aust, Stefanie…[et al.]. EV-Associated MMP9 in High-Grade Serous Ovarian Cancer Is Preferentially Localized to Annexin V-Binding EVs. Disease Markers. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1152624

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1152624