In Vitro Anti-Inflammatory Activity of Morus alba L. Stem Extract in LPS-Stimulated RAW 264.7 Cells
المؤلفون المشاركون
Soonthornsit, Nattaporn
Pitaksutheepong, Chetsadaporn
Hemstapat, Warinkarn
Utaisincharoen, Pongsak
Pitaksuteepong, Tasana
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2017، العدد 2017 (31 ديسمبر/كانون الأول 2017)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2017-06-08
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Morus alba L., also known as white mulberry or Mhon, has long been used in traditional medicines.
This study was aimed to investigate anti-inflammatory activities of mulberry stem ethanolic extract (MSE) in lipopolysaccharide- (LPS-) stimulated RAW 264.7 macrophage cell line.
The MSE was first prepared and then investigated for cell viability using the MTT assay.
The anti-inflammatory activities were investigated through the inhibition of inducible nitric oxide synthase (iNOS), cyclooxygenase- (COX-) 2 mRNA expression, and iNOS protein expression using reverse transcription-polymerase chain reaction (RT-PCR) assay and immunoblotting analysis, respectively.
The inhibition of nitric oxide production of the MSE was also investigated using the Griess reaction assay.
The MSE concentration ranging from 10 to 40 µg/ml yielded cell viability higher than 80%.
The MSE at concentrations of 20 and 40 µg/ml demonstrated anti-inflammatory activity through the inhibition of nitric oxide production via suppression of both the iNOS mRNA and protein.
It was also found to inhibit the expression of COX-2 mRNA in LPS-induced RAW 264.7 cells.
This study is the first to report the anti-inflammatory potential of the extract prepared from the stem of mulberry.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Soonthornsit, Nattaporn& Pitaksutheepong, Chetsadaporn& Hemstapat, Warinkarn& Utaisincharoen, Pongsak& Pitaksuteepong, Tasana. 2017. In Vitro Anti-Inflammatory Activity of Morus alba L. Stem Extract in LPS-Stimulated RAW 264.7 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1153600
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Soonthornsit, Nattaporn…[et al.]. In Vitro Anti-Inflammatory Activity of Morus alba L. Stem Extract in LPS-Stimulated RAW 264.7 Cells. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1153600
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Soonthornsit, Nattaporn& Pitaksutheepong, Chetsadaporn& Hemstapat, Warinkarn& Utaisincharoen, Pongsak& Pitaksuteepong, Tasana. In Vitro Anti-Inflammatory Activity of Morus alba L. Stem Extract in LPS-Stimulated RAW 264.7 Cells. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1153600
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1153600
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر