Radixin Relocalization and Nonmuscle α-Actinin Expression Are Features of Remodeling Cardiomyocytes in Adult Patients with Dilated Cardiomyopathy
المؤلفون المشاركون
Cetinkaya, Ayse
Berge, Benedikt
Sen-Hild, Bedriye
Troidl, Kerstin
Gajawada, Praveen
Kubin, Natalia
Valeske, Klaus
Schranz, Dietmar
Akintürk, Hakan
Schönburg, Markus
Kubin, Thomas
Choi, Yeong-Hoon
Richter, Manfred
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-07-22
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Background.
Pediatric patients show an impressive capacity of cardiac regeneration.
In contrast, severely deteriorated adult hearts do usually not recover.
Since cardiac remodeling—involving the expression of fetal genes—is regarded as an adaptation to stress, we compared hearts of adult patients suffering from dilated cardiomyopathy (DCM) with remodeling of cultured neonatal (NRC) as well as adult (ARC) rat cardiomyocytes and the developing postnatal myocardium.
Methods.
NRC and ARC were stimulated with serum and cardiac morphogens derived from DCM hearts.
Protein synthesis (PS) as well as protein accumulation (PA) was measured, and cell survival was determined under ischemic conditions.
Fetal markers were investigated by Western blot.
Biomarkers of remodeling were analyzed in controls, DCM, and 2- to 6-month-old children with tetralogy of Fallot as well as in neonatal and adult rats by immunofluorescence.
Results.
In NRC, serum and morphogens strongly stimulated PS and PA and the reestablishment of cell-cell contacts (CCC).
In ARC, both stimulants increased PS and CCC, but PA was only elevated after serum stimulation.
In contrast to serum, morphogen treatment resulted in the expression of fetal genes in ARC as determined by nonmuscle α-actinin-1 and α-actinin-4 expression (NM-actinins) and was associated with increased survival under ischemia.
NM-actinins were present in cardiomyocytes of DCM in a cross-striated pattern reminiscent of sarcomeres as well as in extensions of the area of the intercalated disc (ID).
NM-actinins are expressed in NRC and in the developing heart.
Radixin staining revealed remodeling of the area of the ID in DCM almost identical to stimulated cultured ARC.
Conclusions.
Remodeling was similar in ARC and in cardiomyocytes of DCM suggesting evolutionary conserved mechanisms of regeneration.
Despite activation of fetal genes, the atrophy of ARC indicates differences in their regenerative capacity from NRC.
Cardiac-derived factors induced NM-actinin expression and increased survival of ischemic ARC while circulating molecules were less effective.
Identification of these cardiac-derived factors and determination of their individual capacity to heal or damage are of particular importance for a biomarker-guided therapy in adult patients.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cetinkaya, Ayse& Berge, Benedikt& Sen-Hild, Bedriye& Troidl, Kerstin& Gajawada, Praveen& Kubin, Natalia…[et al.]. 2020. Radixin Relocalization and Nonmuscle α-Actinin Expression Are Features of Remodeling Cardiomyocytes in Adult Patients with Dilated Cardiomyopathy. Disease Markers،Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1154217
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cetinkaya, Ayse…[et al.]. Radixin Relocalization and Nonmuscle α-Actinin Expression Are Features of Remodeling Cardiomyocytes in Adult Patients with Dilated Cardiomyopathy. Disease Markers No. 2020 (2020), pp.1-14.
https://search.emarefa.net/detail/BIM-1154217
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cetinkaya, Ayse& Berge, Benedikt& Sen-Hild, Bedriye& Troidl, Kerstin& Gajawada, Praveen& Kubin, Natalia…[et al.]. Radixin Relocalization and Nonmuscle α-Actinin Expression Are Features of Remodeling Cardiomyocytes in Adult Patients with Dilated Cardiomyopathy. Disease Markers. 2020. Vol. 2020, no. 2020, pp.1-14.
https://search.emarefa.net/detail/BIM-1154217
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1154217
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر