Inhibitory Activity of Boesenbergia rotunda (L.)‎ Mansf. Rhizome towards the Expression of Akt and NF-KappaB p65 in Acetic Acid-Induced Wistar Rats

المؤلفون المشاركون

Lesmana, Ronny
Rosdianto, Aziiz Mardanarian
Puspitasari, Irma Melyani
Levita, Jutti

المصدر

Evidence-Based Complementary and Alternative Medicine

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-13، 13ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-05-20

دولة النشر

مصر

عدد الصفحات

13

التخصصات الرئيسية

الطب البشري

الملخص EN

Ethnopharmacological Relevance.

Boesenbergia rotunda has been traditionally used to reduce stomach discomfort in Indonesia.

The objective of the present study was to investigate the anti-inflammatory mechanism and acute toxicity of the ethanol extract of B.

rotunda rhizome (BREE) in Wistar rats.

Materials and Methods.

Forty-eight male Wistar rats were divided into anti-inflammatory mechanism study (n = 18) and acute toxicity study (n = 30).

The anti-inflammatory mechanism study employed six groups (n = 3), e.g., the normal control, negative control, positive control (quercetin 20 mg/kg BW), and three doses of BREE (250 mg/kg BW; 500 mg/kg BW; 1000 mg/kg BW).

All groups (except the normal control) were inflammatory-induced i.p.

using 0.1 mL of 1% of acetic acid.

The expression of Akt and NF-kappaB p65 in the stomach and intestine of the rats was examined using Western blot analysis.

The acute toxicity study (21 days) was conducted by following the Regulation of Indonesia National Agency of Drug and Food Control No.

7/2014 about In Vivo Nonclinical Toxicity Study using 5 doses of BREE (250 mg/kg BW; 500 mg/kg BW; 1000 mg/kg BW; 2000 mg/kg BW; 4000 mg/kg BW).

Results.

BREE reduces the infiltration of inflammatory cells in both the stomach and the intestine of acetic acid-induced rats.

BREE also alters the expression of Akt and NF-kappaB p65 in the rat’s stomach and intestine (p=0.005).

The acute toxicity study reveals no lethal effects and behavioral signs of toxicity at all tested doses, which indicates that the LD50 is greater than 4000 mg/kg BW.

Conclusion.

Taken together, BREE could inhibit the expression of Akt and NF-kappaB p65 in the stomach and intestine of acetic acid-induced Wistar rats.

This plant could be further explored for its potential as plant-based antistomach ulceration.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Rosdianto, Aziiz Mardanarian& Puspitasari, Irma Melyani& Lesmana, Ronny& Levita, Jutti. 2020. Inhibitory Activity of Boesenbergia rotunda (L.) Mansf. Rhizome towards the Expression of Akt and NF-KappaB p65 in Acetic Acid-Induced Wistar Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1157125

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Rosdianto, Aziiz Mardanarian…[et al.]. Inhibitory Activity of Boesenbergia rotunda (L.) Mansf. Rhizome towards the Expression of Akt and NF-KappaB p65 in Acetic Acid-Induced Wistar Rats. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1157125

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Rosdianto, Aziiz Mardanarian& Puspitasari, Irma Melyani& Lesmana, Ronny& Levita, Jutti. Inhibitory Activity of Boesenbergia rotunda (L.) Mansf. Rhizome towards the Expression of Akt and NF-KappaB p65 in Acetic Acid-Induced Wistar Rats. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1157125

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1157125