Highly Sensitive Amperometric α-Ketoglutarate Biosensor Based on Reduced Graphene Oxide-Gold Nanocomposites
المؤلفون المشاركون
Peng, Gang
Yu, Yadong
Chen, Xiaojun
Huang, He
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-08-01
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Herein, a rapid and highly sensitive amperometric biosensor for the detection of α-ketoglutarate (α-KG) was constructed via an electrochemical approach, in which the glutamate dehydrogenase (GLUD) was modified on the surface of reduced graphene oxide-gold nanoparticle composite (rGO-Aunano composite).
The rGO-Aunano composite was one-step electrodeposited onto glassy carbon electrode (GCE) surface and was characterized by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and electrochemical techniques.
In addition, the rGO-Aunano/GCE was also found to electrocatalyze the oxidation of β-nicotinamide adenine dinucleotide (NADH) at the peak potential of 0.3 V, which was negatively shifted compared with that at bare GCE or Aunano/GCE, illustrating better catalytic performance of rGO-Aunano.
After the modification of GLUD, the GLUD/rGO-Aunano/GCE led to effective amperometric detection of α-KG through monitoring the NADH consumption and displayed a linear response in the range of 66.7 and 494.5 μM, with the detection limit of 9.2 μM.
Moreover, the prepared GLUD/rGO-Aunano/GCE was further evaluated to be highly selective and used to test α-KG in human serum samples.
The recovery and the RSD values were calculated in the range of 97.9–102.4% and 3.8–4.5%, respectively, showing a great prospect for its real application.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Peng, Gang& Yu, Yadong& Chen, Xiaojun& Huang, He. 2020. Highly Sensitive Amperometric α-Ketoglutarate Biosensor Based on Reduced Graphene Oxide-Gold Nanocomposites. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1167653
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Peng, Gang…[et al.]. Highly Sensitive Amperometric α-Ketoglutarate Biosensor Based on Reduced Graphene Oxide-Gold Nanocomposites. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1167653
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Peng, Gang& Yu, Yadong& Chen, Xiaojun& Huang, He. Highly Sensitive Amperometric α-Ketoglutarate Biosensor Based on Reduced Graphene Oxide-Gold Nanocomposites. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1167653
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1167653
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر