Development and Validation of UPLC-MSMS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application
المؤلفون المشاركون
Qiu, Xiang-jun
Li, Shuang-long
Zhu, Yong-liang
Zhang, Yi
Liu, Shu-han
Wang, Xiang-die
المصدر
International Journal of Analytical Chemistry
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-03-31
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
In our research, a straightforward UPLC-MS/MS method, with diazepam as the internal standard (IS), was proposed and acknowledged to determine the concentrations of enasidenib in rat plasma.
When preparing the sample, we used acetonitrile for protein precipitation.
The gradient elution method was used, and the mobile phase was acetonitrile and 0.1% formic acid.
Diazepam was used as the IS.
We used the Acquity UPLC BEH C18 column to separate enasidenib and IS.
Under the positive ion electrospray ionization (ESI) source conditions, the mass transfer pairs of enasidenib were monitored by multiple reaction monitoring (MRM) to be m/z 474.2 ⟶ 456.1 and m/z 474.2 ⟶ 267.0, and the IS mass transfer pairs were m/z 285.0 ⟶ 154.0.
Enasidenib had good linearity (r2 = 0.9985) in the concentration range of 1.0–1000 ng/mL.
Besides, the values of intraday and interday precision were 2.25–8.40% and 3.94–5.46%, respectively, and the range of the accuracy values varied from −1.44 to 2.34%.
Matrix effect, extraction recovery, and stability were compliant with FDA approval guidelines in terms of bioanalytical method validation.
We had established a new method that had been applied to the pharmacokinetic study of enasidenib in rats.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Shuang-long& Zhu, Yong-liang& Zhang, Yi& Liu, Shu-han& Wang, Xiang-die& Qiu, Xiang-jun. 2020. Development and Validation of UPLC-MSMS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167658
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Shuang-long…[et al.]. Development and Validation of UPLC-MSMS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1167658
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Shuang-long& Zhu, Yong-liang& Zhang, Yi& Liu, Shu-han& Wang, Xiang-die& Qiu, Xiang-jun. Development and Validation of UPLC-MSMS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167658
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1167658
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر