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High-Efficiency DNA Extraction Using Poly(4,4′-Cyclohexylidene Bisphenol Oxalate)-Modified Microcrystalline Cellulose-Magnetite Composite
المؤلفون المشاركون
Yusof, Nor Azah
Mohammad, Faruq
Al balawi, Aisha Nawaf
Kamaruzaman, Sazlinda
Wasoh, H.
al-Lohedan, Hamad A.
المصدر
International Journal of Polymer Science
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-06-04
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
In this study, we studied the DNA extraction capability of poly(4,4′-cyclohexylidene bisphenol oxalate) following the surface modification and composite formation with that of microcrystalline cellulose (MCC) and magnetic iron oxide nanoparticles (NPs).
The physical characterization techniques like scanning electron microscopy (SEM), Fourier-transform infrared (FTIR) spectroscopy, energy-dispersive X-ray analysis (EDX), and thermogravimetric analysis (TGA) were employed for the poly(bisphenol Z oxalate)-MCC-magnetite composite during different stages of its formation.
The results confirmed the successful modification of the polymer surface.
On testing in the presence of three types of binding buffers, a high value of 72.4% (out of 10,000 ng/μL) efficiency with a total yield of DNA at 2×106 ng and absorbance ratio of A260/A280 (1.980) was observed for the 2 M GuHCl/EtOH binding buffer.
These results were compared against the other two buffers of phosphate-buffered saline (PBS) and NaCl.
The lowest value of DNA extraction efficiency at 8125 ng/μL of 58.845% with absorbance ratios of A260/A280 (1.818) for PBS was also observed.
The study has concluded an enhancement in the DNA extraction efficiency when the polymer is in the composite stage along with cellulose and magnetite particles as compared against the bare polymer.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Al balawi, Aisha Nawaf& Yusof, Nor Azah& Kamaruzaman, Sazlinda& Mohammad, Faruq& Wasoh, H.& al-Lohedan, Hamad A.. 2019. High-Efficiency DNA Extraction Using Poly(4,4′-Cyclohexylidene Bisphenol Oxalate)-Modified Microcrystalline Cellulose-Magnetite Composite. International Journal of Polymer Science،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1168123
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Al balawi, Aisha Nawaf…[et al.]. High-Efficiency DNA Extraction Using Poly(4,4′-Cyclohexylidene Bisphenol Oxalate)-Modified Microcrystalline Cellulose-Magnetite Composite. International Journal of Polymer Science No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1168123
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Al balawi, Aisha Nawaf& Yusof, Nor Azah& Kamaruzaman, Sazlinda& Mohammad, Faruq& Wasoh, H.& al-Lohedan, Hamad A.. High-Efficiency DNA Extraction Using Poly(4,4′-Cyclohexylidene Bisphenol Oxalate)-Modified Microcrystalline Cellulose-Magnetite Composite. International Journal of Polymer Science. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1168123
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1168123
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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