“Turn-On” Fluorescent Assay of Biothiols Based on Nitrogen-Rich Polymer Carbon Nanostrips and Its Application in Cell Imaging
المؤلفون المشاركون
Meng, Xiang-ying
Yi, Zhengjun
Qiao, Jinjuan
Zhao, Ronglan
Chu, Hairong
Wang, Ying
Chen, Xiangyu
المصدر
العدد
المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2019-01-15
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
In this work, a sensitive and selective turn-on fluorimetric method has been developed for the determination of biothiols based on blocking Ag+-induced fluorescence quenching of nitrogen-rich polymer carbon nanostrips (NRPCNSs).
Ag+ ion can induce the fluorescence quenching of NRPCNSs due to the formation of nonfluorescent coordination complexes via robust Ag-N interaction.
Once addition of biothiols, such as cysteine (Cys) and glutathione (GSH), Ag+ ions prefer to interact with biothiols rather than NRPCNSs, which could be attribute to the formation of Ag-S bond, thus leading to effective fluorescent recovery of NRPCNSs.
Under the optimized conditions, excellent linear relationships existed between the recovery degree of the NRPCNSs and the concentrations of Cys and GSH in the range of 0.05 μM to 10 μM and 0.2 μM to 30 μM, respectively.
And, the limits of detection (LODs) for Cys and GSH are 16.5 nM and 65.1 nM, respectively.
The detection system also shows high selectivity against other non-thiol amino acids.
Moreover, the potential in practical applications of this proposed method has been demonstrated by detecting biothiols in human serum and fluorescence imaging of biothiols in living cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Meng, Xiang-ying& Qiao, Jinjuan& Zhao, Ronglan& Chu, Hairong& Wang, Ying& Chen, Xiangyu…[et al.]. 2019. “Turn-On” Fluorescent Assay of Biothiols Based on Nitrogen-Rich Polymer Carbon Nanostrips and Its Application in Cell Imaging. Journal of Chemistry،Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1170775
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Meng, Xiang-ying…[et al.]. “Turn-On” Fluorescent Assay of Biothiols Based on Nitrogen-Rich Polymer Carbon Nanostrips and Its Application in Cell Imaging. Journal of Chemistry No. 2019 (2019), pp.1-12.
https://search.emarefa.net/detail/BIM-1170775
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Meng, Xiang-ying& Qiao, Jinjuan& Zhao, Ronglan& Chu, Hairong& Wang, Ying& Chen, Xiangyu…[et al.]. “Turn-On” Fluorescent Assay of Biothiols Based on Nitrogen-Rich Polymer Carbon Nanostrips and Its Application in Cell Imaging. Journal of Chemistry. 2019. Vol. 2019, no. 2019, pp.1-12.
https://search.emarefa.net/detail/BIM-1170775
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1170775
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر