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Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell
المؤلفون المشاركون
Zhang, Chengshuo
Zhang, Jialin
Jiao, Ao
Li, Feng
Lv, W.
Chen, Baomin
المصدر
International Journal of Endocrinology
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-20
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Cholinergic neurons can functionally support pancreatic islets in controlling blood sugar levels.
However, in islet transplantation, the level of cholinergic reinnervation is significantly lower compared to orthotopic pancreatic islets.
This abnormal reinnervation affects the survival and function of islet grafts.
In this study, the cholinergic reinnervation of beta cells was simulated by 2D and 3D coculture of INS-1 and NG108-15 cells.
In 2D culture conditions, 20 mM glucose induced a 1.24-fold increase (p<0.0001) in insulin secretion from the coculture group, while in the 3D culture condition, a 1.78-fold increase (p<0.0001) in insulin secretion from heterotypic pseudoislet group was observed.
Glucose-stimulated insulin secretion (GSIS) from 2D INS-1 cells showed minimal changes when compared to 3D structures.
E-cadherin expressed in INS-1 and NG108-15 cells was the key adhesion molecule for the formation of heterotypic pseudoislets.
NG108-15 cells hardly affected the proliferation of INS-1 cells in vitro.
Heterotypic pseudoislet transplantation recipient mice reverted to normoglycemic levels faster and had a greater blood glucose clearance compared to INS-1 pseudoislet recipient mice.
In conclusion, cholinergic cells can promote insulin-secreting cells to function better in vitro and in vivo and E-cadherin plays an important role in the formation of heterotypic pseudoislets.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiao, Ao& Li, Feng& Zhang, Chengshuo& Lv, W.& Chen, Baomin& Zhang, Jialin. 2018. Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell. International Journal of Endocrinology،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1171024
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiao, Ao…[et al.]. Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell. International Journal of Endocrinology No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1171024
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiao, Ao& Li, Feng& Zhang, Chengshuo& Lv, W.& Chen, Baomin& Zhang, Jialin. Simulated Cholinergic Reinnervation of β (INS-1) Cells: Antidiabetic Utility of Heterotypic Pseudoislets Containing β Cell and Cholinergic Cell. International Journal of Endocrinology. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1171024
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1171024
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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