Molecular Typing and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus Isolated from Bovine Milk in Tanzania
المؤلفون المشاركون
Hounmanou, Y. M. G.
Mohammed, J.
Ziwa, Michael Henry
Kisanga, Adela
Tuntufye, Huruma Nelwike
المصدر
International Journal of Microbiology
العدد
المجلد 2018، العدد 2018 (31 ديسمبر/كانون الأول 2018)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2018-03-12
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Methicillin-resistant Staphylococcus aureus (MRSA) in raw milk can be transmitted from animals to humans, and in Tanzania raw milk is sold in local markets and consumed as purchased.
This study was performed to determine the molecular characteristics and antimicrobial susceptibility pattern of MRSA strains isolated from raw bovine milk sold at local markets in Tanzania.
A total of 117 raw milk samples were cultured on Baird-Parker medium to isolate S.
aureus and PCR was used for amplification of gltB gene for S.
aureus identification and the presence of mecA gene for methicillin-resistant strains.
Coagulase-negative (CN) S.
aureus were reconfirmed using tube coagulase, DNase, and API Staph tests.
MRSA isolates were spa typed whereas antimicrobial susceptibility testing was performed by the disc diffusion method.
Forty-six coagulase positives (CP) and two CN S.
aureus were identified.
Most strains were resistant to penicillin (72%), and 3 isolates: 2 CN S.
aureus and 1 coagulase-negative Staphylococci (CNS), were phenotypically resistant to vancomycin, oxacillin, and cefoxitin and were confirmed to carry mecA.
Resistance to clindamycin, trimethoprim-sulfamethoxazole, and tetracycline was 23.9%, 30.4%, and 41.3%, respectively.
Twelve isolates exhibited multidrug resistance; however, only one mecA positive strain among the three was typeable and belonged to spa type t2603.
This study reports for the first time the presence of CN variant of MRSA, which was assigned the spa type t2603, and the presence of multidrug resistant S.
aureus isolates from bovine milk in Morogoro, Tanzania.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Mohammed, J.& Ziwa, Michael Henry& Hounmanou, Y. M. G.& Kisanga, Adela& Tuntufye, Huruma Nelwike. 2018. Molecular Typing and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus Isolated from Bovine Milk in Tanzania. International Journal of Microbiology،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1173324
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mohammed, J.…[et al.]. Molecular Typing and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus Isolated from Bovine Milk in Tanzania. International Journal of Microbiology No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1173324
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Mohammed, J.& Ziwa, Michael Henry& Hounmanou, Y. M. G.& Kisanga, Adela& Tuntufye, Huruma Nelwike. Molecular Typing and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus Isolated from Bovine Milk in Tanzania. International Journal of Microbiology. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1173324
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1173324
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر