Development of a Simple and Powerful Analytical Method for Formaldehyde Detection and Quantitation in Blood Samples

المؤلفون المشاركون

Kim, Yong-Hyun
Park, Jeongsik

المصدر

Journal of Analytical Methods in Chemistry

العدد

المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2020-12-30

دولة النشر

مصر

عدد الصفحات

9

التخصصات الرئيسية

الكيمياء

الملخص EN

Human beings are easily exposed to formaldehyde (FA) in a living environment.

Entry of FA into the human body can have adverse effects on human health, depending on the FA concentration.

Thus, a quantitative analysis of FA in blood is necessary in order to estimate its effect on the human body.

In this study, a simple and rapid analytical method for the quantitation of FA in blood was developed.

The total analysis time, including the pretreatment procedure, was less than 20 min.

To ensure a stable analysis, blood samples were stabilized using tripotassium ethylenediaminetetraacetic acid solution, and FA was selectively derivatized using 2,4-dinitrophenylhydrazine as pretreatment procedures.

The pretreated samples were analyzed using a high-performance liquid chromatography-UV system, which is the most common choice for analyzing small-molecule aldehydes like formaldehyde.

Verification of the pretreatment methods (stabilization and derivatization) using FA standards confirmed that the pretreatment methods are highly reliable in the calibration range 0.012–5.761 ng μL–1 (slope = 684,898, R2 = 0.9998, and limit of detection = 0.251 pg·μL–1).

Analysis of FA in the blood samples of a Yucatan minipig using the new method revealed an average FA concentration of 1.98 ± 0.34 ng μL–1 (n = 3).

Blood samples spiked with FA standards were analyzed, and the FA concentrations were found to be similar to the theoretical concentrations (2.16 ± 0.81% difference).

The method reported herein can quantitatively analyze FA in blood at a sub-nanogram level within a short period of time and is validated for application in blood analysis.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Kim, Yong-Hyun& Park, Jeongsik. 2020. Development of a Simple and Powerful Analytical Method for Formaldehyde Detection and Quantitation in Blood Samples. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1174887

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Kim, Yong-Hyun& Park, Jeongsik. Development of a Simple and Powerful Analytical Method for Formaldehyde Detection and Quantitation in Blood Samples. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1174887

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Kim, Yong-Hyun& Park, Jeongsik. Development of a Simple and Powerful Analytical Method for Formaldehyde Detection and Quantitation in Blood Samples. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1174887

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1174887