A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine

المؤلفون المشاركون

Zhou, Rui
Liu, Yangke
Zhang, Jiang
Wang, Shuai
Guo, Yong
He, Tao

المصدر

Journal of Immunology Research

العدد

المجلد 2019، العدد 2019 (31 ديسمبر/كانون الأول 2019)، ص ص. 1-7، 7ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2019-12-01

دولة النشر

مصر

عدد الصفحات

7

التخصصات الرئيسية

الأحياء

الملخص EN

Sublancin is a glycosylated antimicrobial peptide produced by Bacillus subtilis 168 possessing antibacterial and immunomodulatory activities.

This study was aimed at investigating the effects of sublancin on immune functions and serum antibody titer in specific pathogen-free (SPF) broiler chickens vaccinated with Newcastle disease (ND) vaccine.

For this purpose, 3 experiments were performed.

Experiment 1: SPF broiler chicks (14 days old) were randomly allotted to 1 of 7 groups including a blank control (BC), vaccine control (VC), and 5 (3-7) vaccinated and sublancin supplemented at 5, 15, 30, 45, and 60 mg activity/L of water, respectively.

Vaccinated groups (2-7) were vaccinated with ND vaccine by intranasal and intraocular routes at the 14th day.

On 7, 14, 21, and 28 days post vaccination (dpv), the blood samples were collected for the determination of serum hemagglutination inhibition (HI) antibody titer.

Experiment 2: SPF broiler chicks were divided into 1 of 3 groups, i.e., blank control (BC), vaccine control (VC), and sublancin treatment (ST).

On 7, 14, and 21 dpv, the blood samples were collected for measuring HI antibody titer by micromethod.

Experiment 3: the design of this experiment was the same as that of experiment 2.

On 7 and 21 dpv, pinocytosis of peritoneal macrophages, B lymphocyte proliferation assay, measurement of CD4+ and CD8+ T cells, and serum cytokine quantitation were carried out.

It was noted that sublancin promoted B lymphocyte proliferation, increased the proportion of CD8+ T lymphocyte subpopulations, and enhanced the antibody titer in broiler chickens.

In addition, it was also observed that sublancin has the potential to induce the secretion of IFN-γ, IL-10, and IL-4.

In conclusion, these findings suggested that sublancin could promote both humoral and cellular immune responses and has the potential to be a promising vaccine adjuvant.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Liu, Yangke& Zhang, Jiang& Wang, Shuai& Guo, Yong& He, Tao& Zhou, Rui. 2019. A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine. Journal of Immunology Research،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1175639

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Liu, Yangke…[et al.]. A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine. Journal of Immunology Research No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1175639

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Liu, Yangke& Zhang, Jiang& Wang, Shuai& Guo, Yong& He, Tao& Zhou, Rui. A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine. Journal of Immunology Research. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1175639

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1175639