Estimation of Total Quercetin and Rutin Content in Malus domestica of Nepalese Origin by HPLC Method and Determination of Their Antioxidative Activity
المؤلفون المشاركون
Pandey, Jitendra
Bastola, Tonking
Tripathi, Julu
Tripathi, Milu
Rokaya, Rabindra Kumar
Dhakal, Bhawana
D. C., Rabin
Bhandari, Ravin
Poudel, Amrit
المصدر
العدد
المجلد 2020، العدد 2020 (31 ديسمبر/كانون الأول 2020)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2020-11-03
دولة النشر
مصر
عدد الصفحات
13
الملخص EN
Malus domestica (Apple) is one of the most widely cultivated cash crops of Nepal.
Jumla and Mustang are two major pocket areas for the production of apple.
Flavonoids including quercetin and rutin are potent antioxidants present in apples.
This study was designed to quantify and compare the presence of quercetin and rutin in different plant parts (peel, leaf, and bark) among various cultivars of Malus domestica from two pocket zones of Nepal.
A new HPLC-UV method was developed and validated for the quantification of quercetin and rutin.
Polyphenols, flavonoids, and carbohydrate contents were determined by colorimetric methods.
2,2′-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay was carried out to measure in vitro antioxidative activity.
Acid hydrolysis of each extract was carried out by the standard method to measure aglycone quercetin content after hydrolysis of its glycosides.
The total rutin content ranged from 3.69 ± 1.34 to 374.50 ± 2.35 mg/100g dry extract weight.
Before the acidic hydrolysis, the total quercetin content ranged from 2.96 ± 0.13 to 171.05 ± 0.95 mg/100g dry extract weight whereas its amount increased highly after the hydrolysis and it ranged from 80.84 ± 19.65 to 7445.32 ± 29.25 mg/100g dry extract weight.
Total polyphenol content ranged from 19.48 ± 0.23 to 123.48 ± 1.84 µg gallic acid equivalent/mg of dry extract weight.
Similarly, flavonoid content ranged from 2.21 ± 0.72 µg to 755.54 ± 1.91 µg quercetin equivalent/mg of dry extract weight.
Total carbohydrate content ranged from 144.15 ± 3.73 to 484.65 ± 2.63 µg glucose equivalent per 0.5 mg dry extract weight.
All the extracts showed the various degrees of antioxidant activity in a dose-dependent manner.
Among them, stem bark of the Jonathan Jumla showed potent antioxidant activity with IC50 value of 13.003 µg/mL.
The present study provides the information about variation of the phytochemical content among the different cultivars, parts, and geographic locations.
Furthermore, it revealed that bark of Malus domestica cultivars had high quercetin and rutin content with high antioxidant activity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pandey, Jitendra& Bastola, Tonking& Tripathi, Julu& Tripathi, Milu& Rokaya, Rabindra Kumar& Dhakal, Bhawana…[et al.]. 2020. Estimation of Total Quercetin and Rutin Content in Malus domestica of Nepalese Origin by HPLC Method and Determination of Their Antioxidative Activity. Journal of Food Quality،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1184927
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pandey, Jitendra…[et al.]. Estimation of Total Quercetin and Rutin Content in Malus domestica of Nepalese Origin by HPLC Method and Determination of Their Antioxidative Activity. Journal of Food Quality No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1184927
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pandey, Jitendra& Bastola, Tonking& Tripathi, Julu& Tripathi, Milu& Rokaya, Rabindra Kumar& Dhakal, Bhawana…[et al.]. Estimation of Total Quercetin and Rutin Content in Malus domestica of Nepalese Origin by HPLC Method and Determination of Their Antioxidative Activity. Journal of Food Quality. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1184927
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1184927
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر